不同水分条件对玉米根际微生物群落的影响

发布时间：2018-04-11 00:10

本文选题：玉米根际 + 微生物群落　；参考：《作物杂志》2017年01期

【摘要】：通过玉米盆栽试验研究不同水分条件下(土壤孔隙含水量20%和80%)土壤微生物群落结构及种群数量的变化。采集玉米生长第60天时的根际土和表层土,通过Mi Seq Illumina高通量测序技术扩增细菌和古菌16S r RNA基因分析群落多样性,同时通过绝对定量PCR扩增细菌和古菌16S r RNA基因分析其种群数量。结果表明:高通量测序共得到41 546个细菌OTUs和6 921个古菌OTUs。土壤中细菌的多样性丰富,优势菌群为变形菌(Proteobacteria)和酸杆菌(Acidobacteria),而古菌的群落组成单一,优势菌群为奇古菌(Thaumarchaeota)。非度量多维尺度(NMDS)分析表明不同土壤区域中细菌群落结构具有明显差异,而古菌群落结构受水分和土壤区域的影响不明显。定量分析结果显示细菌和古菌数量受土壤区域和水分条件的影响显著。表层土中细菌和古菌数量均显著高于根际土。根际土中细菌和古菌数量在水分充足条件下均显著高于干旱条件,表层土中细菌和古菌数量也表现出相同趋势。本研究结果表明土壤不同区域(生态位)可能是影响土壤中微生物群落最重要的因素。
[Abstract]:The changes of soil microbial community structure and population number under different water conditions (soil pore water content 20% and soil pore water content 80%) were studied by pot experiment of maize.The rhizosphere soil and topsoil of maize were collected at the 60th day of growth. The 16s r RNA gene of bacteria and archaea were amplified by Mi Seq Illumina high throughput sequencing technique.At the same time, the 16s r RNA gene of bacteria and Archaea were amplified by absolute quantitative PCR.The results showed that 41 546 bacterial OTUs and 6 921 ancient bacteria OTUs were obtained by high throughput sequencing.The dominant bacteria in soil were Proteobacteria (Proteobacteria) and Acidobacteria acidobacteriae, while the community composition of ancient bacteria was single, and the dominant flora was Thaumarchaeotaa.Non-metric multi-dimensional scale (NMDS) analysis showed that there were significant differences in the structure of bacterial communities in different soil regions, while the structure of paleomycete communities was not affected by water and soil regions.The results of quantitative analysis showed that the quantity of bacteria and archaea was significantly affected by soil area and water condition.The number of bacteria and archaea in surface soil was significantly higher than that in rhizosphere soil.The number of bacteria and archaea in rhizosphere soil was significantly higher than that in dry soil under the condition of sufficient water, and the number of bacteria and archaea in surface soil showed the same trend.The results show that different soil regions (niche) may be the most important factors affecting soil microbial community.
【作者单位】：西南科技大学生命科学与工程学院;中国农业科学院生物技术研究所;
【基金】：国家973计划(2015CB755701) 国家自然科学基金(31400439) 转基因重大专项(2014ZX0800301B)
【分类号】：S154.3;S513

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