水稻纹枯病生防菌的筛选与鉴定

发布时间：2018-04-11 03:27

本文选题：水稻纹枯病 + 生防菌筛选　；参考：《沈阳农业大学学报》2017年06期

【摘要】：从辽宁省、吉林省和黑龙江省多个地区采集水稻田土样。采用3点平板对峙培养法、菌丝生长速率法和菌核萌发抑制法,综合筛选对水稻纹枯病菌有生防作用的生防菌。结果表明:从土壤中分离获得的211株生防菌中筛选出抑菌活性较高的1株真菌JLF6-2和3株细菌JLN3-2、SYN6-3及JLN3-8。4株生防菌株抑菌圈分别为38.30mm、18.30mm、33.30mm和22.50mm;菌丝生长抑制率分别为77.30%、63.29%、80.35%和100%;菌核萌发抑制率分别为86.67%、83.33%、80.00%和61.35%。经离体试验于接种水稻纹枯病菌前后不同时间喷施供试生防菌发酵滤液,结果对水稻纹枯病均有较高的防治效果。在保护处理中,生防菌株的防效为59.65%~94.92%,其中JLN3-2在24h的防效为94.92%;在治疗处理中,生防菌株的防效为59.32%~74.58%,其中JLN3-2在8h的防效为74.58%。经形态特征、生理生化特性和分子生物学鉴定结果表明:菌株JLF6-2为比莱青霉(Penicillium bilaiae);JLN3-2为纺锤形赖氨酸芽孢杆菌(Lysinibacillus fusiformis);SYN6-3为解淀粉芽孢杆菌(Bacillus amyloliquefaciens);JLN3-8为侧孢短芽孢杆菌(Brevibacillus laterosporus)。
[Abstract]:Soil samples from paddy fields were collected from Liaoning Province, Jilin Province and Heilongjiang Province.Three point plate culture method, hyphal growth rate method and sclerotia germination inhibition method were used to screen biocontrol bacteria against Rhizoctonia solani.The results showed that one fungus JLF6-2, three strains JLN3-2SYN6-3 and three JLN3-8.4 biocontrol strains were selected from 211 strains of biocontrol, the inhibitory rates of mycelium growth were 77.30mm, 63.295mm and 80.35%, respectively, and the inhibitory circles of JLN3-8.4 strains were 38.30mm, 18.30mm, 33.30mm and 22.50mm, respectively, and the inhibition rates of mycelium growth were 77.30mm, 63.295mm and 80.35%, respectively.The inhibition rates of sclerotia germination were 86.67% and 61.35% respectively.After inoculation with Rhizoctonia solani in vitro, the fermentation filtrate was sprayed at different time before and after inoculation of rice sheath blight. The results showed that it had higher control effect on rice sheath blight.In the treatment of protection, the control effect of biocontrol strain was 59.65 ~ 94.922, in which the control effect of JLN3-2 in 24 hours was 94.92, and that of biocontrol strain in treatment was 59.32 ~ 74.58, among which the control effect of JLN3-2 at 8 h was 74.58.
【作者单位】：沈阳农业大学植物保护学院;
【基金】：国家水稻产业技术体系项目(CARS-01) 271%E5%8F%B7)" target="_blank"> 辽宁水稻产业技术体系项目(辽农科<2013>271号)
【分类号】：S435.111.42;S476.1

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