大庆市蚊发生规律及白僵菌对淡色库蚊毒力研究

发布时间：2018-04-13 10:06

  本文选题：大庆 + 蚊　； 参考：《黑龙江八一农垦大学》2015年硕士论文

【摘要】：通过研究大庆市蚊种类、生物学特性、发生规律及球孢白僵菌对幼虫的防治效果，为大庆蚊子的实际防治工作提供理论依据。利用灯诱法、网捕法捕获大庆市成蚊，统计数量，鉴定种类；室外定点定时调查成蚊的发生规律；实验室构建淡色库蚊种群，观察生物学特性，研究温度对其生长发育影响，以及球孢白僵菌不同孢悬液浓度、不同孢悬液温度对淡色库蚊的毒力。 2014年4~10月对大庆市成蚊进行调查，鉴定蚊种类有4属11种，按蚊属（Anopheles）有中华按蚊(Anopheles sinensis)、米塞按蚊(Anopheles messeae)；库蚊属（Culex）有淡色库蚊(Culex pipiens pallens)、凶小库蚊(Culex modestus)、迷走库蚊(Culex vagans)、三带喙库蚊(Culex tritaeniorhynchus)；伊蚊属（Aedes）有背点伊蚊(Aedes dorsalis)、刺痛伊蚊(Aedes excrucians)、叮刺伊蚊(Aedes sticticus)、黑海伊蚊(Aedes cyprius)；脉毛蚊属（Culiseta）有日本脉毛蚊(Culiseta nippoaica)。大庆市广泛分布蚊种为淡色库蚊、凶小库蚊、迷走库蚊、背点伊蚊。优势蚊种淡色库蚊占总捕获数量的49.81%，背点伊蚊占总捕获数量的18.15%。大庆市蚊始见期为4月20日，终见期为10月5日。7~8月是蚊数量发生高峰期，占总捕获数的65.98%。4~7月优势蚊种为淡色库蚊，8月优势蚊种为凶小库蚊，9~10月优势蚊种为背点伊蚊。 实验室构建了淡色库蚊种群，饲养的关键技术在于严格控制温度、光照时间和保持水质清洁。明确了淡色库蚊的雌蚊吸血习性、产卵习性、卵孵化习性等。淡色库蚊的卵在15~35℃下均可正常孵化，随着温度提高卵孵化时间缩短。幼虫发育历期随着温度的升高而缩短，15℃时，，从1龄到羽化成虫需要23.62d，35℃时，为7.82d，30℃时，死亡率为40%，35℃时，死亡率达80%。 采用不同浓度、不同温度的球孢白僵菌孢子悬浮液处理不同虫龄、不同密度的淡色库蚊幼虫。结果表明，球孢白僵菌孢悬液浓度与幼虫死亡率呈正相关，相关系数为0.9947；同一孢子浓度处理下，幼虫死亡率随虫口密度增加显著提高；孢悬液对不同虫龄的致死能力差异显著；相同孢子浓度下，4龄幼虫的致死率明显低于1~3龄幼虫；白僵菌孢悬液对幼虫致死率随温度升高显著提高。
[Abstract]:By studying the species, biological characteristics, occurrence law and control effect of Beauveria bassiana on larvae in Daqing city, the theoretical basis for the actual control of mosquitoes in Daqing was provided.The adult mosquitoes in Daqing city were captured by lamp trap and netting method. The number of mosquitoes was counted and the species was identified. The occurrence rule of adult mosquitoes was investigated at fixed points outside. The population of Culex pipiens pallens was constructed in laboratory to observe the biological characteristics and to study the effect of temperature on its growth and development.And the virulence of Culex pipiens pallens with different suspension concentration and temperature of Beauveria bassiana.The adult mosquitoes in Daqing City were investigated from April to October of 2014, and 11 species of mosquitoes belonging to 4 genera were identified.Sticticus, Aedes priusus; Culiseta) have the genus Culiseta nippoaicia.Daqing City widely distributed species of Culex pipiens pallens, Culex pipiens, Culex vaginalis, Aedes dorsalis.The dominant mosquito species, Culex pipiens pallens, accounted for 49.81% of the total captured number, and Aedes dorsalis accounted for 18.15%.The blood-sucking habits, spawning habits and egg hatching habits of Culex pipiens pallens were clarified.The eggs of Culex pipiens pallens can hatch normally at 1535 鈩

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