胶孢炭疽菌CgRGS2基因的克隆及生物学功能

发布时间：2018-04-17 09:47

本文选题：胶孢炭疽菌 + G蛋白信号调控因子　；参考：《微生物学报》2017年01期

【摘要】：【目的】G蛋白信号调控因子(Regulators of G-protein signaling,RGS)是G蛋白的一类负调控因子,在植物病原菌生长发育及致病过程中发挥着重要的作用,然而目前还未有关于胶孢炭疽菌RGS蛋白生物学功能的研究。本试验的目的是克隆胶孢炭疽菌的一个RGS基因CgRGS2,并分析其生物学功能。【方法】利用PCR技术扩增CgRGS2的基因并进行生物信息学分析,利用同源重组的方法获得CgRGS2基因的敲除突变体,并在突变体的基础上获得互补株,通过表型分析确定该基因的生物学功能。【结果】通过PCR扩增获得了CgRGS2的基因,其编码一个574个氨基酸的蛋白,在N末端含有一个RGS功能域。该基因敲除突变体同野生型相比,表现为营养生长缓慢,气生菌丝浓密,分生孢子产量降低且孢子呈多端萌发,对氧化压力及SDS敏感,致病性减弱等。【结论】CgRGS2蛋白参与调控胶孢炭疽菌的营养生长,分生孢子产量及萌发,氧化应激反应及细胞壁完整性,对其致病性也具有一定的影响。
[Abstract]:[objective] G protein signal regulators of G-protein signaling (RGS) is a negative regulator of G protein, which plays an important role in the growth, development and pathogenicity of plant pathogens.However, there is no study on the biological function of RGS protein of Bacillus anthracis.The purpose of this study was to clone CgRGS2, a RGS gene of Bacillus anthracis, and to analyze its biological function. [methods] CgRGS2 gene was amplified by PCR and bioinformatics was performed.The knockout mutants of CgRGS2 gene were obtained by homologous recombination, and complementary strains were obtained on the basis of mutants. The biological function of the gene was determined by phenotypic analysis. [results] the gene of CgRGS2 was amplified by PCR.It encodes a 574 amino acid protein and contains a RGS functional domain at the N terminal.Compared with wild type, the knockout mutant showed slow vegetative growth, dense hyphal density, decreased conidial yield and multi-terminal germination of spores, and was sensitive to oxidative pressure and SDS.[conclusion] CgRGS2 protein is involved in the regulation of vegetative growth, conidial yield and germination, oxidative stress response and cell wall integrity of anthrax, and has a certain effect on its pathogenicity.
【作者单位】：海南大学环境与植物保护学院;
【基金】：国家自然科学基金(31560045) 海南省自然科学基金(20153132)~~
【分类号】：S432.44

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