盐碱胁迫对黄瓜嫁接苗根际土壤细菌和真菌群落结构及丰度的影响

发布时间：2018-04-21 18:10

本文选题：盐碱胁迫 + 黄瓜嫁接　；参考：《中国生态农业学报》2017年11期

【摘要】：为了解盐碱胁迫对黄瓜嫁接苗根际土壤细菌和真菌群落结构的影响,本研究以2种耐盐碱砧木‘华砧108’(T1)、‘神力铁木砧’(T2)和2种盐碱敏感砧木‘辉太郎’(S1)、‘京欣砧6号’(S2)为试材,自根苗作为对照,以混合盐(盐分摩尔比为NaHCO_3∶Na_2SO_4∶NaCl∶Na_2CO_3=4∶2∶2∶0.15)浓度为100 mmol·L~(-1)、pH 9.0的处理液处理20 d、30 d、40 d(定植30 d、40 d、50 d),利用PCR-DGGE技术,研究了盐碱胁迫对不同砧木嫁接的黄瓜幼苗根际土壤微生物群落结构和丰度的影响。结果表明,耐盐碱的砧木品种T1、T2根际土壤真菌DGGE图谱条带数显著高于盐碱敏感的S2和自根苗对照CK,并且耐盐碱的品种T2土壤细菌的Shannon-Wiener指数与均匀度指数均显著高于盐碱敏感的品种S1、S2和自根苗对照CK。耐盐碱品种T1的细菌16S rDNA基因拷贝数在定植50 d时显著高于盐碱敏感的品种及自根苗;在定植40 d时,耐盐碱的砧木T2真菌ITS基因拷贝数显著高于盐碱敏感的品种以及黄瓜自根苗;定植50 d时,耐盐碱的砧木真菌ITS基因拷贝数显著高于盐碱敏感的品种,但与自根苗差异不显著。不同耐盐碱性砧木嫁接黄瓜幼苗根际土壤微生物群落结构组成和丰度存在差异。以上研究表明,随着盐碱胁迫时间的增加,耐盐碱性不同的砧木嫁接苗根际土壤微生物群落丰度与结构多样性产生了较大差异,间接改变了土壤微生态环境,致使土壤微生物数量和丰富度也发生改变。耐盐碱的砧木品种可能通过改善土壤微环境来加强其自身的耐盐碱特性。
[Abstract]:In order to understand the effects of salt and alkali stress on the structure of bacteria and fungi community in the rhizosphere of cucumber grafted seedlings, In this study, two saline-alkali tolerant rootstocks, '108T1HU' and two saline-alkali-sensitive rootstocks, 'Huatarang' S1S1''Jingxin anvil No. 6 (S2) were used as test materials, and self-rooted seedlings were used as control, and the results showed that the rootstocks were sensitive to salt and alkali-sensitive rootstocks. The mixture salt (NaHCO _ 3: NaH _ 2SO _ 4: NaCl: Na _ 2CO _ 3 / T _ 4: NaCl: Na _ 2CO _ 3: 4: 2: 2: 0.15) was treated with a concentration of 100 mmol / L ~ (-1) / pH 9.0 for 20 ~ (30) d ~ ~ ~ (40) d ~ (40) d ~ (40 d) (30 d ~ (30 d) ~ 40 d ~ (50) d), and the PCR-DGGE technique was used. The effects of salt and alkali stress on microbial community structure and abundance in rhizosphere soil of cucumber seedlings grafted with different rootstocks were studied. The results show that The number of DGGE bands of saline-alkali tolerant rootstock variety T _ 1 / T _ 2 rhizosphere soil fungi was significantly higher than that of saline-alkali sensitive S _ 2 and self-rooted seedling CK, and the Shannon-Wiener index and evenness index of saline-alkali tolerant variety T _ 2 soil bacteria were significantly higher than that of saline-alkali sensitive species. S _ 1 / S _ 2 and self-rooting seedling control CK. The copy number of 16s rDNA gene of saline-alkali tolerant variety T1 was significantly higher than that of saline-alkali sensitive variety and self-rooted seedling at 50 days, and at 40 days after planting, the number of copies of 16s rDNA gene was significantly higher than that of saline-alkali sensitive variety and self-rooted seedling. The copy number of ITS gene of saline-alkali tolerant rootstock T2 fungi was significantly higher than that of saline-alkali sensitive variety and cucumber self-rooted seedling, and the ITS gene copy number of saline-alkali tolerant rootstock fungus was significantly higher than that of saline-alkali sensitive variety after 50 days of planting, but there was no significant difference with self-root seedling. The composition and abundance of microbial community in rhizosphere soil of cucumber seedlings grafted with different saline-alkali tolerant rootstocks were different. The results showed that, with the increase of saline-alkali stress time, the abundance and structure diversity of soil microbial communities of rootstocks with different saline-alkali tolerance varied greatly, which indirectly changed the soil microecological environment. The amount and richness of soil microbes also changed. Salt-tolerant rootstocks may enhance their saline-alkali tolerance by improving soil microenvironment.
【作者单位】：东北农业大学园艺园林学院;
【基金】：国家大宗蔬菜产业技术体系专项(CARS-25-08)资助~~
【分类号】：S154.3;S642.2

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