辽西油松侧柏混交林对土壤微生物群落功能多样性的影响

发布时间：2018-04-24 08:16

本文选题：微生物功能多样性 + 碳源利用　；参考：《沈阳农业大学学报》2017年01期

【摘要】：为探讨油松(Pinus tabulaeformis)、侧柏(Platycladus orientalis)混交林造林模式对土壤微生物群落功能多样性的影响,以朝阳市建平县白山林场试验区油松纯林(YC)、无植物种植土壤(NP)和油松-侧柏二者的混交林(YB)为研究对象,运用Biolog-Eco技术,对不同造林模式土壤微生物代谢功能多样性进行研究,对不同造林模式土壤微生物群落功能多样性进行分析。结果表明:不同造林模式土壤微生物群落代谢活性差异显著,总碳源的利用能力(AWCD)的顺序为:YBYCNP,油松、侧柏混交林土壤微生物代谢最快,活性最强。3种造林模式土壤微生物对6大碳源的利用率存在差异,混交林土壤微生物对L-天门冬酰胺、L-苯基丙氨酸、吐温40、吐温80、i-赤藓糖醇、D-甘露醇和4-羟基苯甲酸7种碳源的利用代谢能力显著高于油松纯林(p0.05)。Biolog代谢指纹图谱分析表明混交林土壤微生物对吐温80的利用能力最高,AWCD值为1.6537;油松纯林土壤微生物对L-精氨酸的利用能力最高,AWCD值为1.547。主成分分析显示,YB和YC土壤微生物群落具有相似的碳源利用模式,土壤微生物群落代谢功能相似。3种造林模式土壤微生物Shannon指数(H)和Simpson指数(D)无明显差异,物种Mclntosh指数(U)依次为YBYCNP。油松和侧柏混交后可以提高土壤微生物活性。由此可见,在辽西半干旱区营造油松-侧柏混交林是可行的。
[Abstract]:In order to study the effect of afforestation model of Pinus tabulaeformis and Platycladus orientalis on the functional diversity of soil microbial communities, Using pure Pinus tabulaeformis forest and mixed forest of Pinus tabulaeformis and Platycladus tabulaeformis in the experimental area of Baishan Forest Farm in Jianping County of Chaoyang City as the research object, the microbial metabolic function diversity of different afforestation models was studied by using Biolog-Eco technique. The functional diversity of soil microbial communities in different afforestation models was analyzed. The results showed that the metabolic activities of soil microbial communities in different afforestation models were significantly different, and the order of total carbon utilization capacity was:: YBYCNP, Pinus tabulaeformis and Platycladus orientalis mixed forest. There were differences in the utilization of 6 major carbon sources among soil microbes in the most active afforestation model, and there was a significant difference in the utilization of L- asparagine and L- phenylalanine in mixed forest soil microbes. The utilization and metabolism ability of seven carbon sources of Tween 40, Tween 80 i- erythritol Dmannitol and 4-hydroxybenzoic acid were significantly higher than those of pure Pinus tabulaeformis forest p0.05N. Biolog fingerprinting analysis showed that soil microbes in mixed forest had the highest utilization capacity of Tween 80. The value of AWCD was 1.6537, and that of soil microorganism in pure forest of Pinus tabulaeformis was 1.547. The principal component analysis showed that the soil microbial communities of YB and YC had similar carbon source utilization patterns, but the metabolic function of soil microbial communities was similar to that of 3.The soil microbial Shannon index (H) and the Simpson index (D) had no significant difference. The Mclntosh index of species was YBYCNP. When mixed with Pinus tabulaeformis and Platycladus orientalis, soil microbial activity could be improved. Thus, it is feasible to construct mixed forest of Pinus tabulaeformis and Platycladus orientalis in semi-arid area of western Liaoning.
【作者单位】：沈阳农业大学土地环境学院;沈阳农业大学林学院;
【基金】：国家林业局林业公益性行业专项面上项目(201304216);国家林业局林业公益性行业专项重点项目(201404303-05) 辽宁省博士科研启动基金项目(2014106)
【分类号】：S714.3

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