两株烟草黑胫病拮抗菌的筛

发布时间：2018-04-28 13:29

本文选题：细菌 + 烟草黑胫病　；参考：《中国烟草科学》2017年03期

【摘要】：烟草黑胫病在我国烟区分布广泛,严重影响烟草的产量和质量。筛选出对烟草具有防病和促生作用的细菌是有效控制烟草黑胫病危害和提高烟草产量的重要途径。通过稀释涂布平板法、对峙培养法从烟草病株根际土壤中筛选出YBM-4和YJC-4两株细菌。根据分子形态学、最适生长条件和生理生化及16S r DNA基因序列分析比对结果,鉴定YBM-4是枯草芽孢杆菌(Bacillus subtilis),YJC-4是沙福芽孢杆菌(Bacillus safensis)。在对峙培养中,YBM-4和YJC-4细菌对烟草黑胫病菌丝的相对抑制率分别是85.34%和72.99%。在盆栽试验中,YBM-4和YJC-4对烟株生物量和农艺性状的促生作用明显,YBM-4、YJC-4和YBM-4+YJC-4混剂(比例1:1)对烟草黑胫病的平均防治效果分别是76.37%、80.06%和81.50%,病程进展曲线下面积(AUDPC)分别比对照降低265.01,281.58,287.32。表明细菌YBM-4和YJC-4具有潜在的利用和开发价值。
[Abstract]:Tobacco black shank disease is widely distributed in tobacco regions in China, which seriously affects the yield and quality of tobacco. It is an important way to effectively control the harm of black shank disease and increase the yield of tobacco by screening the bacteria which can prevent and promote the growth of tobacco. Two strains of bacteria YBM-4 and YJC-4 were isolated from the rhizosphere soil of tobacco plant by dilution coating plate method and confrontation culture method. According to the results of molecular morphology, optimum growth conditions, physiological and biochemical conditions and 16s r DNA gene sequence alignment, YBM-4 was identified as Bacillus subtilisa subtilis YJC-4 as Bacillus safensis. The relative inhibition rates of YBM-4 and YJC-4 on tobacco filaments were 85.34% and 72.99%, respectively. In pot experiment, the effects of YBM-4 and YJC-4 on biomass and agronomic characters of tobacco plants were obviously promoted. The average control effects of YBM-4 YJC-4 and YBM-4 YJC-4 mixture (ratio 1: 1) on tobacco black shank disease were 76.370.06% and 81.50%, respectively, and the area under the progression curve was 265.01281.58c287.32 lower than that of the control. The results indicate that the bacteria YBM-4 and YJC-4 have potential utilization and development value.
【作者单位】：中国农业科学院烟草研究所;中国农业科学院研究生院;贵州省烟草公司贵阳市公司;四川省烟草公司攀枝花市公司;
【基金】：贵州省烟草公司贵阳市公司重大专项“贵阳烟草主要病虫草绿色防控技术集成示范与推广”(201602) 中国烟草总公司四川省公司科技项目“烟草主要病虫害绿色防控技术研究与应用”(SCYC201604)
【分类号】：S435.72;S476.1

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