吡虫啉对蜜蜂幼虫的室内毒性研究

发布时间：2018-04-29 04:39

本文选题：吡虫啉 + 蜜蜂幼虫　；参考：《生态毒理学报》2016年04期

【摘要】：吡虫啉是目前使用最广泛的新烟碱类农药,其不合理使用破坏蜜蜂的嗅觉记忆能力,影响采集行为,导致多种亚致死效应。利用室内人工饲养蜜蜂幼虫技术,选取意大利蜜蜂幼虫为研究对象,经3 d适应性饲养后,连续3 d喂食含不同浓度的吡虫啉日粮,测定蜜蜂幼虫的存活率、化蛹率和羽化率,评估吡虫啉对蜜蜂幼虫生长发育影响。结果表明,吡虫啉处理剂量为15ng·幼虫~(-1)时,并不影响蜜蜂幼虫存活率、化蛹率和羽化率;当处理剂量达到150 ng·幼虫~(-1)时,蜜蜂幼虫羽化率下降,但不影响蜜蜂幼虫的存活率和化蛹率;幼虫7 d半数致死剂量为2 300 ng·幼虫~(-1)。高剂量吡虫啉对蜜蜂幼虫存活率、化蛹率和羽化率均存在影响,处理剂量越高,存活率、化蛹率和羽化率越低,呈高度负相关(r=-0.99,-0.94,-0.89),同时蜜蜂幼虫的羽化时间延长。研究可为吡虫啉的合理使用提供科学参考。
[Abstract]:Imidacloprid (imidacloprid) is one of the most widely used nicotinic pesticides at present. Its irrational use destroys the ability of honeybee's olfactory memory and affects the collection behavior, resulting in many sublethal effects. The artificial rearing of honeybee larvae was carried out in the laboratory. After 3 days of adaptive feeding, the larvae of Italian bees were fed with different concentrations of imidacloprid diet for 3 days. The survival rate, pupation rate and emergence rate of bee larvae were measured. To evaluate the effect of imidacloprid on the growth and development of honeybee larvae. The results showed that the survival rate, pupation rate and emergence rate of honeybee larvae were not affected by imidacloprid treatment dose of 15ng larvae, but the emergence rate of honeybee larvae decreased when the dose reached 150ng larvae. However, the survival rate and pupation rate of honeybee larvae were not affected, and the LD50 of larvae was 2 300 ng / d. High dose imidacloprid had an effect on the survival rate, pupation rate and emergence rate of bee larvae. The higher the treatment dose, the lower the survival rate, the lower the pupation rate and the emergence rate, and the higher the negative correlation was. The emergence time of bee larvae was prolonged. The study can provide scientific reference for the rational use of imidacloprid.
【作者单位】：环境保护部南京环境科学研究所;南京信息工程大学江苏省大气环境与装备技术协同创新中心;
【基金】：2015年中央级公益性科研院所基本科研业务专项(农药对蜜蜂慢性毒性、幼虫毒性试验方法及配套技术研究)
【分类号】：X171.5;X592

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