广藿香内生真菌多样性及其对青枯菌的拮抗活性

发布时间：2018-04-30 07:46

本文选题：广藿香 + 内生真菌　；参考：《微生物学通报》2017年05期

【摘要】：【目的】对广藿香各部位内生真菌的类群结构及多样性进行系统分析,筛选出对青枯菌具拮抗活性的菌株。【方法】采用组织块分离法从广藿香健康植株的根、茎、叶中分离内生真菌,结合形态学和基于ITS-r DNA序列分析鉴定方法分析广藿香内生真菌的类群结构及多样性,并利用双层平板拮抗法筛选对青枯菌具有抑制活性的菌株。【结果】从广藿香中共分离获得313株内生真菌,隶属于30个属,其中链格孢属(占28.75%)、拟茎点霉属(占23.00%)和炭疽菌属(11.82%)为优势类群,此外还分离到炭皮菌属、弯孢聚壳属、Gibellulopsis、新萨托菌属、葡萄座腔菌属、篮状菌属等较为少见的类群。茎、叶内生真菌的定殖率和分离率明显高于根,而根部多样性指数为2.64,要高于茎(2.00)和叶(1.97);广藿香茎与叶、根与茎、根与叶之间的内生真菌相似性指数分别为0.35、0.20、0.19,均小于0.5,显示各部位之间的内生真菌组成不相似程度高。从313株广藿香内生真菌中通过拮抗实验筛选到16株对青枯菌有拮抗活性的菌株,其中GHXR07、GHXR27和GHXR29的拮抗活性尤为显著,分别被鉴定为Talaromyces sp.、Myrothecium roridum Tode和Talaromyces wortmannii(Kl?cker)Benjamin。【结论】广藿香内生真菌具有丰富的物种多样性且其类群分布具有一定的组织特异性,其中部分菌株对青枯菌具有明显的拮抗活性。
[Abstract]:[Objective] to systematically analyze the group structure and diversity of Endophytic Fungi from different parts of patchouli, and to screen out the antagonistic strains of the Rhizoctonia. [Methods] the isolation of Endophytes from the roots, stems and leaves of the healthy plants of patchouli was separated by tissue block method, and the methods were analyzed based on the morphology and ITS-r DNA sequence analysis and identification methods. The group structure and diversity of the endophytic fungi were screened by double plate antagonistic method. [results] 313 endophytic fungi were isolated from Herba patchouli, which belong to 30 genera, including the spores of 28.75%, the Pseudomonas sp. (23%) and the anthrax (11.82%) as the dominant groups. It is also isolated from the genus charcoal, polycline, Gibellulopsis, new Sarto, vineyard, and basket like fungi. The colonization rate and separation rate of stem and leaf endophytic fungi are obviously higher than those of root, and the root diversity index is 2.64, higher than that of stems (2) and leaves (1.97); Patchouli stems and leaves, roots and stems, roots and leaves. The similarity index of endophytic fungi was 0.35,0.20,0.19, respectively, less than 0.5, which showed that the composition of endophytic fungi was not similar. From 313 strains of patchouli endophytic fungi, 16 strains were screened by antagonistic experiments, and the antagonistic activities of GHXR07, GHXR27 and GHXR29 were particularly significant, respectively. Talaromyces sp., Myrothecium roridum Tode and Talaromyces wortmannii (Kl? Cker) Benjamin. [Conclusion] endophytic fungi of patchouli have abundant species diversity and their group distribution has a certain tissue specificity, some of which have obvious antagonistic activity to the bacterial blight.

【作者单位】：广东药科大学中药学院;广东药科大学基础学院广东省生物活性药物研究重点实验室;
【基金】：国家自然科学基金项目(No.31400111) 广东省自然科学基金项目(No.S2013040016110)~~
【分类号】：S435.672;S476.1

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