川芎内生放线菌活性菌的筛选及鉴定

发布时间：2018-04-30 08:55

本文选题：川芎 + 内生放线菌　；参考：《江苏农业科学》2017年03期

【摘要】：为了了解川芎内生放线菌抑制常见致病菌的能力,从中筛选活性较强的放线菌资源,采用纸片扩散法测定发酵提取液对5种指示菌的抑菌活性,结合经典分类法和分子生物学法鉴定活性菌种。结果表明,对金黄色葡萄球菌(Staphylococcus aureus)、枯草芽孢杆菌(Bacillus subtilis)、大肠杆菌(Escherichia coli)、黑曲霉(Aspergillus niger)和白色念球菌(Canidia albicans)表现抑制活性的菌株占川芎内生放线菌的百分比分别为83.33%、76.67%、23.33%、30.00%、13.33%;从中选出4株活性较强的放线菌,Blast比对结果表明,菌株F3、F19、F-L-5、F-L-7的16S r DNA序列与Gen Bank已知序列链霉菌属金色类群[Streptomyces sp.(KF126314.1)]、暗灰链霉菌[Streptomyces canus(AB7184118.1)]、肿痂链霉菌[Streptomyces turgidiscabies(FJ883749.1)]、暗色产色链霉菌[Streptomyces phaeochromogenes(KP718589.1)]高度相似,同源性分别为99%、100%、99%、99%。综合鉴定,F3为链霉菌属金色类群,F19为暗灰链霉菌,F-L-5为肿痂链霉菌,F-L-7为暗色产色链霉菌。表明川芎内生放线菌中具有应用潜力的高活性菌株,并鉴定出4株活性菌。
[Abstract]:In order to understand the ability of endophytic actinomycetes to inhibit common pathogenic bacteria, we screened the active Actinomycetes Resources, and used the paper diffusion method to determine the bacteriostasis activity of the 5 indicative bacteria by the paper diffusion method. The results showed that the Staphylococcus aureus (Staphylococcus aure) was identified with the classical classification method and the molecular biology method. Us), the percentage of Endophytic Actinomycetes from Bacillus subtilis (Bacillus subtilis), Escherichia coli (Escherichia coli), Aspergillus niger (Aspergillus Niger) and Candida albicans (Canidia albicans) accounted for 83.33%, 76.67%, 23.33%, 30%, 13.33% of the endophytic actinomycetes, respectively, and 4 strains of actinomycetes were selected and the Blast ratio was compared. The results indicate that the strains F3, F19, F-L-5, F-L-7, 16S R DNA sequence and Gen Bank known sequence Streptomyces aureate [Streptomyces sp. (KF126314.1)]. ] highly similar, the homology is 99%, 100%, 99%, and 99%., respectively. F3 is the golden group of Streptomyces, F19 is Streptomyces acycetes, F-L-5 is Streptomyces swell, and F-L-7 is dark color Streptomyces. It shows the high active strain of the endophytic actinomycetes in Ligusticum chuanxiong, and the identification of 4 active bacteria.

【作者单位】：成都中医药大学/中药资源系统研究与开发利用省部共建国家重点实验室培育基地/中药材标准化省部共建教育部重点实验室;
【基金】：国家自然科学基金(编号:81001610) 高等学校博士学科点专项科研基金(编号:20135132110004) 成都中医药大学科技发展基金(编号:ZRQN1546)
【分类号】：S476.1

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