缙云山林地土壤纤维素分解菌及酶活性研究

发布时间：2018-05-07 23:17

本文选题：纤维素分解菌 + 纤维素酶　；参考：《西北农林科技大学学报(自然科学版)》2017年01期

【摘要】：【目的】研究不同林地土壤纤维素分解菌的数量及酶活性,为高效纤维素分解菌群的筛选提供参考。【方法】以缙云山的楠竹林、马尾松林和杉木林土壤为研究对象,测定其土壤养分含量、纤维素分解菌数量和纤维素酶活性,然后就三者之间的相关性进行分析。【结果】(1)杉木林地的土壤纤维素分解菌数量、相对酶活性、羧甲基纤维素酶活性(Cx)和滤纸酶活性(FPA)平均值均显著高于楠竹林地,分别是后者的9.70,1.24,2.80和2.81倍,且楠竹林地上述各指标均高于马尾松林地,分别是马尾松林地的1.36,1.15,1.03和1.07倍。(2)杉木林地和马尾松林地的Cx、FPA变化动态相似,Cx、FPA均在培养至5d时最高,之后略有下降;而楠竹林地的Cx、FPA则是在5d前平稳增长,之后迅速上升,培养至7d时最高。(3)相对酶活性、Cx、FPA两两呈极显著正相关,纤维素分解菌数量与相对酶活性、Cx、FPA呈极显著正相关,土壤纤维素分解菌数量、相对酶活性均与有机质、有效磷含量呈极显著正相关,Cx与有机质、全氮、全磷、有效磷呈显著正相关,FPA与有机质、全氮、全磷呈显著正相关,与有效磷呈显著负相关。【结论】杉木林地的纤维素分解菌活力较强,纤维素分解转化能力及碳循环速率高于楠竹林地和马尾松林地。
[Abstract]:[Objective] to study the quantity and enzyme activity of cellulose decomposing bacteria in different woodland soils, and to provide reference for the screening of efficient cellulose decomposition bacteria. [Methods] the soil nutrient content, the number of fibrin decomposing bacteria and the cellulase activity of the soil of the bamboo forest, Pinus massoniana forest and Chinese fir forest in Jinyun Mountain were measured, and then three [results] [results] (1) the number of cellulose decomposing bacteria, relative enzyme activity, carboxymethyl cellulase activity (Cx) and filter paper enzyme activity (FPA) in Chinese fir woodland were significantly higher than that of Nanzhu woodland, which were respectively 9.70,1.24,2.80 and 2.81 times of the latter, and the above indexes were higher than that of Masson pine woodland. The 1.36,1.15,1.03 and 1.07 times of the woodland of Pinus massoniana respectively. (2) the changes of the forest land and the Cx of the woodland of Pinus massoniana were similar, and the changes of FPA were similar, and Cx and FPA were the highest when they were cultured to 5D, and then decreased slightly, while the Cx and FPA of the bamboo forest land grew steadily before 5D, and then grew rapidly to 7d. (3) the relative enzyme activity, Cx, FPA 22 showed extremely obvious. There was significant positive correlation between the number of cellulose decomposing bacteria and relative enzyme activity, Cx and FPA. The number of cellulose decomposing bacteria and the relative enzyme activity were significantly positively correlated with the content of organic matter and effective phosphorus. Cx was significantly positively correlated with organic matter, total nitrogen, total phosphorus and effective phosphorus. FPA was positively correlated with organic matter, total nitrogen and total phosphorus, and effective. There is a significant negative correlation of phosphorus. [Conclusion] cellulose decomposing bacteria in Chinese fir woodland is stronger, cellulose decomposition and transformation ability and carbon cycle rate are higher than that of bamboo forest land and Masson pine woodland.

【作者单位】：西南大学资源环境学院;
【基金】：重庆市应用开发计划项目(cstc2013yykfA80014)
【分类号】：S714

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