油松菌根际高效解磷钾细菌筛选与鉴定

发布时间：2018-05-12 01:10

本文选题：油松 + 解磷钾细菌　；参考：《基因组学与应用生物学》2017年04期

【摘要】：本研究筛选出油松-褐环乳牛肝菌菌根根际土中高效解磷钾细菌并对其进行种类鉴定。采用平板稀释法自菌根际土中筛选解磷钾细菌,并用钼蓝比色法和原子吸收法分别对其液体培养5 d后的菌株进行解磷能力和解钾能力测定,筛选出高效的解磷钾细菌。同时,对筛选出的菌株进行显微观察、生物学鉴定和16Sr DNA序列分析。通过筛选得出解有机磷细菌P6和解无机磷细菌P15能力较强,其培养液中有效磷浓度分别为283 mg/L和898.5 mg/L,经鉴定P6属于节杆菌属(Arthrobacter sp.),P15为荧光假单胞菌(Pseudomonas fluorescens),以及2株能力较强的解钾细菌K1和K12,其培养液中有效钾浓度分别为21.6 mg/L,19.3 mg/L,经鉴定K1为壤霉菌属(Agromyces cerinus),K12为中华根瘤菌属(Sinorhizobium sp.)。由此可见,油松-褐环乳牛肝菌菌根根际存在高效解磷钾细菌,褐环乳牛肝菌可以通过影响根际解磷钾细菌的种类及能力来改善油松幼苗的根际磷钾营养状况。
[Abstract]:In this study, the high efficiency potassium phosphate bacteria in rhizosphere soil of Pinus tabulaeformis and brown ring dairy cattle were screened and identified. The phosphorus and potassium bacteria were screened by plate dilution method from mycorrhizal soil, and the phosphorus removal capacity and potassium ability of the strains after 5 d were determined by molybdenum blue colorimetric method and atomic absorption method. At the same time, the screened strains were observed by Microobservation, biological identification and 16Sr DNA sequence analysis. Through screening, it was found that the solution of organophosphorus bacteria P6 and inorganic phosphorus bacteria P15 were stronger, and the effective phosphorus concentration in the medium was 283 mg/L and 898.5 mg/L respectively, and P6 belonged to Arthrobacter sp. (Arthrobacter sp.), P15, P15. For Pseudomonas fluorescens (Pseudomonas fluorescens) and 2 strong potassium solubilizing bacteria K1 and K12, the effective potassium concentration in the culture medium was 21.6 mg/L and 19.3 mg/L respectively. The K1 was identified as the genus of mycorrhizal fungi (Agromyces cerinus) and K12 for the genus Rhizobium (Sinorhizobium sp.). Thus, the existence of the rhizosphere of rhizometa mycorrhizae in Pinus tabulaeformis and brown ring cows The high efficiency phosphate solubilizing bacteria, the bacterium Boletus, can improve the rhizosphere phosphorus and potassium status of Pinus tabulaeformis seedlings by affecting the type and ability of phosphate solubilizing bacteria in rhizosphere.

【作者单位】：内蒙古农业大学生命科学学院;内蒙古农业大学林学院;
【基金】：内蒙古教育厅基金(NJZY14097) 自治区科技计划项目(20120317)共同资助
【分类号】：S791.254

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