基于高通量测序的杨树人工林根际土壤真菌群落结构

发布时间：2018-05-28 18:49

本文选题：高通量测序 + 根序　；参考：《应用生态学报》2017年04期

【摘要】：研究不同根序细根根际土壤微生物群落组成结构对深入了解根系-微生物互作关系具有重要意义.本研究采用Illumina Mi Seq测序平台,对杨树人工林非根际土壤和不同根序细根根际土壤的真菌群落结构进行分析.物种注释结果显示:杨树1~2级根(R1)、3级根(R2)和4~5级(R3)根际及非根际土壤(NR)中分别包含128、124、130和101个真菌属,表明杨树根际存在对真菌群落构建的选择性机制.不同根序根际土壤中相对丰度1%的真菌属有7个,木霉属在1~2级根根际土壤中丰度较高,毛孢子菌属和曲霉属分别是3级根和4~5级根根际土壤中丰度最高的真菌属.α多样性指数表明:根际土壤真菌的多样性在不同根序间存在显著差异,低级根显著高于高级根(P0.05).β多样性指数表明:真菌群落随着序级的升高差异性不断上升,相似性不断降低.不同根序细根根际真菌群落的趋异化组成和结构与细根功能具有密切关系.
[Abstract]:It is important to study the soil microbial community composition structure of different root order fine rhizosphere soil in order to understand the relationship between root and microorganism. In this study, Illumina Mi Seq sequencing platform was used to analyze the community structure of fungi in non-rhizosphere soil and fine rhizosphere soil of poplar plantation. The results of species annotation showed that the rhizosphere and non-rhizosphere soil of poplar R1 / 2 and R1 / R3) contained 128124130 and 101 genera, respectively, indicating that there was a selective mechanism for fungal community construction in poplar rhizosphere. There were 7 fungi genera with relative abundance of 1% in rhizosphere soil of different rhizosphere sequence, while Trichoderma had higher abundance in rhizosphere soil of grade 1 or 2. The genus Cryosporium and Aspergillus were the most abundant fungi genera in rhizosphere soil of grade 3 and grade 4 respectively. A diversity index indicated that the diversity of fungi in rhizosphere soil was significantly different among different rhizosphere sequences. The 尾 -diversity index of lower roots was significantly higher than that of higher ones. 尾 -diversity index indicated that the difference of fungi community increased with the increase of sequence level, and the similarity decreased continuously. The dissimilation composition and structure of different root order fine rhizosphere fungi communities are closely related to fine root function.
【作者单位】：山东农业大学林学院;国家林业局泰山森林生态系统定位研究站;山东省林业科学研究院;
【基金】：国家自然科学基金项目(31570618,31270670) 中国博士后科学基金项目(2015M572070) 山东省博士后创新基金项目史丹利功能性肥料基金项目资助~~
【分类号】：S714.3

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