优化鸡粪微生态分解及添加降解液促进微藻过量合成生物油脂

发布时间：2018-05-31 02:25

本文选题：鸡粪降解液 + 葡萄糖　；参考：《食品与生物技术学报》2017年06期

【摘要】：以小球藻(Chlorella protothecoides)为出发菌株,优化鸡粪微生态分解及添加降解液促进微藻细胞生长和胞内油脂合成。通过控制溶解氧(DO)优化鸡粪微生态分解,获得高氮和低氮两种降解液。微藻培养过程分为细胞生长(0~120 h)和油脂合成(120~200 h)两个阶段,前期添加高氮降解液促进细胞生长,后期添加低氮降解液和葡萄糖补充氮源及能量过量合成油脂,实现细胞浓度和生物油脂产率最大化。C.protothecoides培养期间,0~120 h恒速流加(15 m L/(L·D))高氮降解液,120~200 h流加(15 m L/(L·D))低氮降解液和葡萄糖(1.5 g/(L·D)),培养结束(200h)时,油脂质量浓度高达5.28 g/L,分别比对照及不添加葡萄糖时油脂质量浓度增加74.8%和21.1%。结果表明,两阶段鸡粪降解液添加与葡萄糖再生ATP结合策略促进油脂合成是有效的。
[Abstract]:Chlorella protothecoides (Chlorella protothecoides) was used as the starting strain to optimize the microecological decomposition of chicken manure and to promote the growth of microalgae cells and the synthesis of intracellular fat. The microecological decomposition of chicken manure was optimized by controlling dissolved oxygen (DOO), and two kinds of degradation solution, high nitrogen and low nitrogen, were obtained. The culture process of microalgae was divided into two stages: cell growth (120 h) and oil synthesis (120 h). High nitrogen degradation solution was added to promote cell growth in early stage, low nitrogen degradation solution and glucose supplemented nitrogen source and excessive energy to synthesize oil at later stage. To maximize cell concentration and bio-oil yield. C. protothecoides during 120 h constant flow and 15 mL / L DX) high nitrogen degradation solution (120 h) plus 15 mL / L DX) low nitrogen degradation solution and glucose (1.5 g / L 路L ~ (-1) D _ (1) at the end of 200 h. The oil concentration reached 5.28 g / L, which was 74.8% and 21.1g / L higher than that of the control and without glucose respectively. The results showed that it was effective to promote the synthesis of oil and fat by adding ATP to degrade chicken manure in two stages.
【作者单位】：江苏理工学院化学与环境工程学院;
【基金】：国家自然科学基金项目(21502074) 江苏省产学研前瞻联合性研究项目(BY2015028-04)
【分类号】：TE667;X713

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