茅尾海红树林土壤可培养放线菌多样性及其抗尖孢镰刀菌活性分析

发布时间：2018-05-31 07:35

本文选题：红树林 + 放线菌　；参考：《中国抗生素杂志》2017年04期

【摘要】：目的研究茅尾海红树林植物根际土壤放线菌多样性并进行抗菌活性筛选,为发现放线菌新物种以及新农用生防菌奠定基础。方法采用传统涂布分离方法和复合营养成分培养基,从25份红树林土壤样品中分离放线菌;通过PCR扩增菌株16S r RNA基因序列,并通过构建系统发育树研究放线菌多样性;采用纸片扩散法分析放线菌发酵液和乙酸乙酯萃取浓缩液对2种尖孢镰刀菌菌体拮抗和孢子萌发抑制活性。结果通过排重获得117株放线菌,16S r RNA基因序列比对分析表明117株放线菌分布于6个亚目10个科19个属,除优势菌属链霉菌属外,还发现纤维微球菌属,戈登菌属,栖白蚁菌属,拟诺卡菌属和植物放线孢菌属等稀有放线菌属;尖孢镰刀菌拮抗活性试验表面,共有23株放线菌在至少1个抗菌活性检测中显示为阳性,总阳性率为19.66%。结论广西北部湾茅尾海红树林根际土壤中存在丰富的放线菌资源,具有从中发现新物种和新型农用生防菌的潜力。
[Abstract]:Objective to study the diversity of actinomycetes in rhizosphere soil of Maowihai mangrove plants and screen their antibacterial activities so as to lay a foundation for the discovery of new species of actinomycetes and new agricultural biocontrol bacteria. Methods Actinomycetes were isolated from 25 mangrove soil samples by traditional coating method and compound nutrient medium, the 16s r RNA gene sequence was amplified by PCR, and the diversity of actinomycetes was studied by constructing phylogenetic tree. The inhibitory activities of actinomycetes fermentation broth and ethyl acetate extract concentrate against two species of Fusarium oxysporum were studied by disk diffusion method. Results the 16s r RNA gene sequences of 117 strains of actinomycetes were obtained by sequencing. The results showed that 117 strains of actinomycetes were distributed in 10 families and 19 genera of 6 suborders. In addition to the dominant genus Streptomyces, micrococcus, Gordon's, and termites were also found. Rare actinomycetes such as Nocardia and actinomycetes, and 23 strains of actinomycetes showed positive in at least one antimicrobial activity test, with a total positive rate of 19.66 on the surface of the antagonistic activity test of Fusarium oxysporum. Conclusion there are abundant actinomycetes resources in the rhizosphere soil of Maowihai mangrove in Beibu Bay, Guangxi, which has the potential to find new species and new agricultural biocontrol bacteria.
【作者单位】：广西民族大学海洋与生物技术学院广西高校微生物与植物资源利用重点实验室;广西民族大学化学化工学院;
【基金】：国家自然科学基金(No.31260004,31660005) 南宁市科技局项目(No.20153330) 广西民族大学相思湖青年学者创新团队资助项目(No.201406)
【分类号】：S476.1

【相似文献】