芝麻饼肥中芝麻素和芝麻林素含量的测定

发布时间：2018-06-03 11:17

本文选题：芝麻饼肥 + 芝麻素　；参考：《河南工业大学学报(自然科学版)》2017年03期

【摘要】：利用高效液相色谱法同时测定芝麻饼肥中芝麻素和芝麻林素的含量。液相色谱条件为:流动相为甲醇水溶液(V_(甲醇)∶V_水=80∶20),流速1.0 m L/min,进样量5.0μL,柱温为室温,紫外检测器波长287 nm。检验结果表明:被测两峰完全分离,且峰形较好,芝麻素和芝麻林素均在1~100 mg/L范围内线性关系良好,R~2分别为1.000 0、0.999 9;该方法的精密度、重现性及准确度均较好,芝麻素平均回收率在98.2%~100.2%之间,芝麻林素平均回收率在96.7%~99.8%之间;样品提取液在2 h内稳定。芝麻饼肥中芝麻素和芝麻林素总含量为1.2~133.7 mg/100 g,芝麻素与芝麻林素含量比例为0.9~3.1。试验验证了高效液相色谱法同时测定芝麻饼肥中芝麻素、芝麻林素含量的可行性,为芝麻饼肥的质量检测提供了技术支持。
[Abstract]:The content of sesamin and sesamin in sesame cake fertilizer was determined by HPLC. The liquid chromatography conditions are as follows: the mobile phase is methanol aqueous solution (methanol: v _ water 80: 20), the flow rate is 1.0 mL / min, the injection amount is 5.0 渭 L, the column temperature is room temperature, the UV detector wavelength is 287 nm. The results showed that the two peaks were completely separated, and the peak shape was better. The linear relationship between sesamin and sesamin was 1.000 ~ 0.999 9 in the range of 1 ~ 100 mg/L. The precision, reproducibility and accuracy of the method were good. The average recoveries of sesamin and sesamin were between 98.2% and 100.2%, respectively, and the average recoveries of sesamin and sesamin were 96.7% and 99.8%, respectively, and the sample extract was stable within 2 hours. The total content of sesamin and sesamin in sesame cake fertilizer was 1.2133.7 mg/100 / g, and the ratio of sesamin to sesamin was 0.9g / 3.1.The total content of sesamin and sesamin in sesame cake fertilizer was 1.2133.7 mg/100 / g. The feasibility of simultaneous determination of sesamin and sesamin in sesame cake fertilizer by HPLC was verified, which provided technical support for the quality detection of sesame cake fertilizer.
【作者单位】：河南省产品质量监督检验院;
【基金】：河南省科技计划项目(2016ZJ07)
【分类号】：O657.72;TQ440.72

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