赤霉素信号转导相关基因OsGID1调控水稻对褐飞虱抗性

发布时间：2018-06-10 17:54

本文选题：水稻 + 赤霉素　；参考：《浙江大学》2015年硕士论文

【摘要】：赤霉素(gibberellins, GAs)是一种重要的四环双萜类植物生长激素,在植物生长发育中发挥着重要作用。同时,赤霉素介导的植物生长与植物防御反应之间存在相互制约作用,从而使植物能根据环境要求在生长与防御之间做出平衡与选择。然而,目前对于赤霉素信号途径调控植物防御反应的机理还了解很少。为此,我们克隆了一个水稻赤霉素信号受体基因.-OsGID1;机械损伤、褐飞虱(Nilaparvata lugens, rice brown planthopper, BPH)为害、水杨酸(salicylic acid, SA)处理可诱导该基因的表达,但茉莉酸(jasmonic acid, JA)处理并不产生诱导效应。过表达OsGID1导致水稻SA含量显著降低、乙烯含量显著增加；同时,OsGID1负调控GA合成酶基因OsCPS、OsKAO、 OsGA20ox的转录水平,而正调控GA信号途径抑制因子基因OsDELLA转录水平。生测结果表明,过表达OsGID1导致BPH雌成虫取食与产卵嗜好性降低,并引起BPH若虫孵化率、单雌产卵量均显著下降。上述结果表明,OsGID1在诱导抗虫反应中发挥着重要作用,作用的机理可能与OsGID1影响GA、SA、H2O2以及乙烯等信号途径有关。
[Abstract]:Gibberellins (GAs) is an important growth hormone of dicterpenoids, which plays an important role in plant growth and development. At the same time, gibberellin mediated plant growth and plant defense response exist mutual constraints, so that plants can make the balance and choice between growth and defense according to environmental requirements. However, little is known about the mechanism of gibberellin signaling pathway regulating plant defense response. Therefore, we cloned a rice gibberellin signal receptor gene. -OsGID1; mechanical injury, Nilaparvata lugens, rice brown planthopper, BPH). Salicylic acid (SA) treatment could induce the gene expression, but jasmonic acid (JA) treatment did not induce the gene expression. Overexpression of OsGID1 resulted in a significant decrease in SA content and a significant increase in ethylene content, and a negative regulation of the transcriptional level of the GA synthase genes OsCPS- OsKAOand OsGA20ox, while the positive regulation of the GA signaling pathway inhibitor gene OsDELLA. The results of bioassay showed that overexpression of OsGID1 resulted in the decrease of feeding and spawning habits of female BPH nymphs, the hatching rate of BPH nymphs, and the decrease of oviposition quantity of single female. These results suggest that OsGID1 plays an important role in the induction of insect-resistant reactions, and the mechanism may be related to the effects of OsGID1 on the signaling pathways such as H _ 2O _ 2, H _ 2O _ 2 and ethylene.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S433

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