解有机磷拮抗细菌的筛选及其解磷特性和拮抗作用

发布时间：2018-06-11 12:10

本文选题：解有机磷细菌 + 解磷能力　；参考：《江苏农业学报》2017年04期

【摘要】：为了获得高效解有机磷细菌,从油菜地土壤中分离到4株对卵磷脂有不同降解能力的细菌。解有机磷细菌发酵上清液中有效磷含量为8.59~28.34μg/ml,较CK上清液中有效磷含量增加8.03~27.78μg/ml,其中菌株JYP-3解磷能力最强,其发酵上清液中有效磷含量为28.34μg/ml,比对照增加27.78μg/ml。结合解有机磷细菌的菌落形态特征、生理生化特征和16S rDNA序列分析结果,初步确定菌株JYP-1、JYP-4为枯草芽孢杆菌(Bacillus subtilis),JYP-2、JYP-3为桥石短芽孢杆菌(Brevibacillus choshinensis)。菌株JYP-3对水稻纹枯病菌(Rhizoctonia solani)、烟草青枯病菌(Ralstonia solanacearum)有较强的拮抗作用,对黄瓜疫病菌(Phytophthora melonis)没有拮抗作用。
[Abstract]:In order to obtain highly efficient organophosphorus bacteria, four strains of bacteria with different degradability of lecithin were isolated from rapeseed soil. The available phosphorus content in the fermentation supernatant of organophosphorus bacteria was 8.59 ~ 28.34 渭 g / ml, which was 8.03 ~ 27.78 渭 g / ml higher than that of CK supernatant. The strain JYP-3 had the strongest ability to release phosphorus, and the available phosphorus content in the supernatant was 28.34 渭 g / ml, 27.78 渭 g / ml higher than that of the control. Combined with the colony morphology, physiological and biochemical characteristics of organophosphorus bacteria and the results of 16s rDNA sequence analysis, the strain JYP-1 and JYP-4 was identified as Bacillus subtilisia subtilis JYP-2and JYP-3 as Bacillus choshinensis. Strain JYP-3 had strong antagonism against Rhizoctonia solanii and Ralstonia solanacearum, but had no antagonism against Phytophthora melonis.
【作者单位】：湖南省微生物研究院;
【基金】：湖南省科技计划项目(2015NK3056)
【分类号】：S154.3

【相似文献】