土壤真菌组成对黄土高原梯田种植类型的响应

发布时间：2018-06-13 11:49

本文选题：梯田 + 种植类型　；参考：《中国环境科学》2017年08期

【摘要】：以黄土丘陵沟壑区的梯田化模范县——庄浪县堡子沟流域的3种种植类型(玉米、苹果、苹果与土豆间作)梯田的土壤真菌为对象,运用Illumina Hi Seq高通量测序技术对表层土壤(0~20cm)中真菌r RNA基因的ITS1可变区进行研究,分析了土壤真菌的多样性、群落组成和相对丰度,同时结合土壤基本理化性质探讨了不同种植类型下影响真菌群落组成的因素.结果表明:(1)3种种植类型土壤真菌OTU数在688~862间变化,分属于13个门,87个纲,164个目,Chao1、ACE、Shannon、Simpson指数表现为间作地和苹果地大于玉米地;(2)土壤优势真菌为子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、接合菌门(Zygomycota),子囊菌门的相对丰度在间作地最高,主要受土壤全氮和速效钾的影响,而接合菌门的相对丰度在苹果地最高,受土壤容重的影响较大,有机碳含量对担子菌门的影响较大;(3)不同种植类型梯田之间土壤真菌群落物种多样性相似,但真菌门、纲、种的相对丰度不同,种植类型间因有机肥的施用不同而通过土壤理化性质的变化影响土壤真菌的群落组成,有机肥的施用可提高当地梯田的土壤肥力.
[Abstract]:Three types of soil fungi (maize, apple and potato intercropping) in the terraced terrace in the Loess Hilly and Gully region were studied in Zhuanglang County, Zhuanglang County, China. Illumina Hi Seq high-throughput sequencing technique was used to study the ITS1 variable region of rRNA gene in surface soil. The diversity, community composition and relative abundance of fungi were analyzed. At the same time, combined with the basic physical and chemical properties of soil, the factors affecting the composition of fungal community under different planting types were discussed. The results showed that the number of soil fungi OTU varied from 6888 to 862 in three planting types. They belong to 13 phylum and 87 classes. The Shannon Simpson index of 164 orders Chao1ACEE shows that the soil dominant fungi are Ascomycotataa, Basidiomycotataa, Zygomycotataa, and the relative abundance of Ascomycotaa is the highest in the intercropping field, and the relative abundance of Ascomycotaa is the highest in the intercropping field. It was mainly affected by soil total nitrogen and available potassium, while the relative abundance of conjugate bacteria was the highest in apple field, and was influenced by soil bulk density. The effect of organic carbon content on basidiomycetes was significant. (3) the species diversity of soil fungal communities was similar among terraces of different planting types, but the relative abundance of fungi phylum, class and species were different. The community composition of soil fungi was influenced by the change of soil physical and chemical properties due to the different application of organic fertilizer. The application of organic fertilizer could improve the soil fertility of terrace.
【作者单位】：西北农林科技大学资源环境学院农业部西北植物营养与农业环境重点实验室;
【基金】：水利部公益性行业科研专项经费(201501045) 国家自然科学基金资助项目(41101254)
【分类号】：S154.3

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