上海典型畜禽场周边河流雌激素污染特征研究

发布时间：2018-06-13 12:17

本文选题：雌激素 + SPE-HPLC-MS/MS　；参考：《农业环境科学学报》2017年08期

【摘要】：为了探究畜禽养殖场周边河流中雌激素类物质的污染特征,利用固相萃取-高效液相色谱-串联质谱(SPE-HPLC-MS/MS)方法,对上海市三个典型畜禽养殖场(猪场、鸡场、奶牛场)周边河流上下游地表水中雌激素的种类和含量进行测定。结果显示,天然雌激素E1(雌酮)、E2(雌二醇)和E3(雌三醇)在各个采样点均被检出,浓度范围分别为21.66~73.40、8.75~55.96、4.56~23.90 ng·L~(-1);人工合成雌激素EE2(乙炔基雌二醇)的检出率为99.44%,浓度范围是ND~19.42 ng·L~(-1),DES(己烯雌酚)的检出率为27.78%,浓度范围是ND~3.23 ng·L~(-1)。采用EEQ(雌二醇当量)评价各水体中雌激素类物质的总体雌激素活性,结果显示,所有采样点EEQ的浓度范围为54.15~194.61 ng·L~(-1),在三个养殖场周边河流中,EE2对EEQ的贡献率均为最高。综合分析表明,三个典型畜禽场周边河流均受到不同程度的雌激素污染,且猪场和奶牛场周边河流中雌激素活性较高。典型猪场和典型奶牛场是E2的主要排放源,典型猪场是E1的主要排放源,而典型鸡场对周边河流产生的雌激素污染较小。
[Abstract]:In order to investigate the pollution characteristics of estrogens in rivers around livestock and poultry farms, three typical livestock and poultry farms in Shanghai (pig farm and chicken farm) were studied by SPE-HPLC-MS / MSMS method with solid-phase extraction (SPE-HPLC-MS / MS) method. The species and content of estrogen in the surface water of the upper and lower reaches of the surrounding rivers were determined. The results showed that natural estrogen E _ 1 (estradiol) and E _ 3 (estriol) were detected at all sampling sites. The detection rate of synthetic estrogen EE2 (ethynyl estradiol) was 99.4444. The detection rate of DES (diethylstilbestrol) was 27.78, and the range of synthetic estrogen EE2 (ethynyl estradiol) was 27.78ng / L ~ (-1) ~ (-1) ~ (-1) and 3.23 ng / L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) ~ (-1). EEQ (estradiol equivalent) was used to evaluate the total estrogenic activity of estrogens in various water bodies. The results showed that the concentration range of EEQ in all sampling sites was 54.15 ~ 194.61 ng / L ~ (-1) / L ~ (-1), and the contribution rate of EE2 to EEQ was the highest in the rivers around the three farms. The comprehensive analysis showed that the rivers around the three typical livestock and poultry farms were polluted by estrogen to varying degrees, and the estrogenic activity of the rivers around the pig farms and dairy farms was higher. Typical pig farm and typical dairy farm are the main emission sources of E2, typical pig farm is the main emission source of E1, and typical chicken farm produces less estrogen pollution to the surrounding rivers.
【作者单位】：东华大学环境科学与工程学院;上海市环境科学研究院;华东理工大学资源与环境工程学院;
【基金】：国家自然科学基金青年科学基金项目(21607105) 上海市环保科研项目(2016-2,2016-3)~~
【分类号】：X522;X713

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