阿特拉津降解酶的提取及酶活性分析

发布时间：2018-06-18 15:16

  本文选题：阿特拉津降解酶 + HPLC　； 参考：《北方园艺》2016年14期

【摘要】：以阿特拉津降解菌株为试验材料,采用冰浴超声破壁水提法提取粗酶,用氯仿萃取培养液里残余的阿特拉津,用HPLC测定阿特拉津的残留量,在温度、pH、粗酶液含量不同、培养时间不同的条件下,研究了粗酶对阿特拉津降解率的影响,以期更好地利用阿特拉津降解酶。结果表明:阿特拉津降解酶最适宜的温度为35℃左右,最适pH 7.5;当粗酶液含量达到50%以上时,底物降解比较完全。
[Abstract]:Using atrazine degrading strain as experimental material, the crude enzyme was extracted by ice bath ultrasonic water extraction, and the residual atrazine was extracted by chloroform. The residue of atrazine was determined by HPLC. The content of crude enzyme was different at temperature and pH. The effect of crude enzyme on the degradation rate of atrazine was studied under different culture time in order to make better use of atrazine degrading enzyme. The results showed that the optimum temperature of atrazine degrading enzyme was about 35 鈩，

本文编号：2035931