微生物多样性对土壤氮磷钾转化、酶活性及油菜生长的影响

发布时间：2018-06-19 21:25

本文选题：土壤微生物多样性 + 土壤有效养分　；参考：《生态学报》2016年18期

【摘要】：采用灭菌土壤分别接种不同稀释倍数(1、10~(-2)、10~(-4)和10~(-6))未灭菌土壤悬浊液的方法,研究了土壤微生物多样性降低对油菜生长和养分吸收、土壤养分有效性和酶活性的影响。结果表明:(1)随着接种土壤悬浊液稀释倍数增加,油菜生物量逐渐降低,10-4的油菜生物量显著低于1和10~(-2),10~(-6)仅为1的26%;(2)油菜氮、磷和钾的吸收量与油菜生物量呈现相同的变化规律;(3)土壤铵态氮浓度随接种土壤悬浊液稀释倍数增加而降低;而土壤硝态氮则以10~(-4)为最高,其它处理间没有显著差异;土壤有效磷未发生显著变化;有效钾反而有上升趋势;(4)土壤多酚氧化酶(PhO X)活性随接种土壤悬浊液稀释倍数增加逐渐升高;β-1,4-葡萄糖苷酶(βG)活性以10~(-6)为最高,而其它处理差异不显著;土壤亮氨酸酶氨肽酶(LAP)活性和酸性磷酸酶(AP)活性变化不显著;(5)相关分析表明,油菜生物量与土壤铵态氮浓度的对数显著正相关;与多酚氧化酶、葡萄糖苷酶和亮氨酸氨肽酶活性显著负相关。研究表明,微生物多样性降低主要通过抑制土壤氮素释放影响植物生长。
[Abstract]:The effects of soil microbial diversity on rapeseed growth, nutrient uptake, soil nutrient availability and enzyme activity were studied by inoculating different dilutions of different dilutions (1 ~ 10 ~ 10 ~ (-10) ~ (-2) and 10 ~ (10) ~ (-6) ~ (-1) of unsterilized soil suspensions. The results showed that with the increase of dilution ratio of suspensions in inoculated soil, the biomass of rapeseed decreased gradually. The biomass of Brassica napus 10 ~ (-4) was significantly lower than that of 1 and 10 ~ 10 ~ (2) C ~ (-1). The biomass of rape was only 26 ~ (2) N of 1). The uptake of phosphorus and potassium was the same as that of rape biomass. (3) the concentration of soil ammonium nitrogen decreased with the increase of dilution times of suspensions in inoculated soil, but the nitrate nitrogen in soil was the highest in 10 ~ (10) ~ (-4), but there was no significant difference between other treatments. There was no significant change in available phosphorus in soil, but there was an increasing trend in available potassium. The activity of polyphenol oxidase (PPO) in soil gradually increased with the dilution multiple of soil suspensions, and the activity of 尾 -1 + 4-glucosidase (尾 -G6) was the highest. The results of correlation analysis showed that the biomass of rape was positively correlated with the logarithm of soil ammonium nitrogen concentration, and with polyphenol oxidase. The activities of glucosidase and leucine aminopeptidase were negatively correlated. The results showed that the decrease of microbial diversity affected plant growth mainly by inhibiting soil nitrogen release.
【作者单位】：中国农业科学院农业资源与农业区划研究所农业部植物营养与肥料重点实验室;中国农业科学院农业环境与可持续发展研究所;浙江大学教育部环境修复与生态健康教育部重点实验室;

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