湘南红壤稻田AOA-amoA、narG、nosZ基因丰度及其环境影响因子

发布时间：2018-06-19 22:37

  本文选题：红壤稻田 + 硝化作用　； 参考：《中国土壤与肥料》2017年01期

【摘要】：为了解南方红壤稻田参与土壤氮循环过程的功能微生物及其与环境因子间的普遍规律性关系,应用荧光定量PCR方法研究了稻田中不同时期氨氧化古菌氨单加氧酶编码基因AOA-amoA、硝酸盐还原酶编码基因narG和N_2O还原酶编码基因nosZ基因丰度的变化,结合主成分分析(PCA)、冗余分析(RDA)探讨了有关硝化、反硝化微生物功能基因丰度变化及其与土壤环境因子间的相互关系。结果表明:AOA-amoA、narG和nosZ基因丰度间极显著正相关;AOA-amo A、narG和nosZ基因丰度随水稻生育进程逐渐降低,在早稻成熟期显著低于翻地前及早稻移栽前。土壤有机质、电导率、微生物生物量碳、微生物生物量氮、pH值、Eh及盐酸提取态Fe(Ⅱ)对AOA-amoA、narG和nosZ基因丰度的变化有重要影响,其中盐酸提取态Fe(Ⅱ)与其相关性最强。本研究探讨了红壤稻田氮转化微生物在不同时期的数量变化及其环境影响因素,为合理调控红壤稻田氮转化提供了理论依据。
[Abstract]:In order to understand the functional microbes involved in soil nitrogen cycling in red soil paddy fields in southern China and their general regular relationship with environmental factors. Fluorescence quantitative PCR (FQ-PCR) was used to study the changes of AOA-amo A, narg and NosZ gene abundance of ammonia monooxygenase coding gene, nitrate reductase gene narg and N2O reductase gene in different stages of ammonia-oxidizing palaeomycetes in paddy fields. Combined with principal component analysis (PCAA) and redundancy analysis (RDA), the changes of microbial functional gene abundance of nitrification and denitrification and their relationship with soil environmental factors were discussed. The results showed that the abundance of AOA-amo Aannar G and NosZ genes decreased gradually with the growth of rice, and were significantly lower at maturity stage of early rice than before turning over and transplanting early rice. The results showed that the abundance of AOA-amo Aannar G and NosZ gene was significantly positive correlation between the two genes. Soil organic matter, electrical conductivity, microbial biomass carbon, microbial biomass nitrogen pH value (Eh) and hydrochloric acid extracted Fe (鈪，

本文编号：2041624