高效解磷兼解钾活性菌株分离筛选的初步研究

发布时间：2018-06-20 06:29

本文选题：解磷菌 + 解钾菌　；参考：《扬州大学学报(农业与生命科学版)》2016年01期

【摘要】：将采集的常年施用磷肥和钾肥的土壤样品,经适当稀释后分别涂布于难溶无机磷和难溶无机钾的固体培养基上,分别获得24株解磷菌和15株解钾菌。将获得的解磷菌和解钾菌分别接种到含有难溶无机磷和解钾液体培养基中进行培养,通过测定发酵液中可溶性磷和可溶性钾含量,筛选出5株高效解磷菌和3株高效解钾菌,其中溶磷效果最好的菌株为P-6-1,培养7d后发酵液中可溶性磷增加量达33.07μg·mL~(-1);菌株K-1-1的解钾效果最好,发酵液中可溶性钾增加量可达0.081g·L~(-1)。为获得兼具溶磷和解钾能力的菌株,将24株解磷菌接种到解钾液体培养基中,同时将15株解钾菌接种到难溶无机磷液体培养基中,通过分别测定解磷菌的解钾能力和解钾菌的溶磷能力,成功筛选出2株兼具溶磷和解钾能力的菌株,分别为K-1-1和K-6-4。其中解钾菌K-1-1可使含磷培养基中可溶性磷增加量达6.81μg·mL~(-1)。与目前报道的解磷或解钾菌株相比,菌株K-1-1具有显著优势。
[Abstract]:The soil samples of phosphorus fertilizer and potassium fertilizer applied all year round were coated on the solid medium of insoluble inorganic phosphorus and insoluble inorganic potassium after proper dilution, and 24 and 15 strains of phosphorus releasing bacteria were obtained, respectively. The obtained bacteria were inoculated into the liquid medium containing insoluble inorganic phosphorus and potassium, respectively. Through the determination of the contents of soluble phosphorus and soluble potassium in fermentation broth, 5 strains of high efficiency phosphate bacteria and 3 strains of high efficiency bacteria were screened out. The most effective strain was P-6-1, the soluble phosphorus increased to 33.07 渭 g / mL ~ (-1) and K-1-1 to 0.081 g / L ~ (-1) 路L ~ (-1) ~ (-1) in fermentation broth after 7 days culture. In order to obtain both phosphate and potassium solubilizing bacteria, 24 phosphate releasing bacteria were inoculated into potassium hydrolyzing liquid medium and 15 potassium hydrolysate bacteria were inoculated into insoluble inorganic phosphorus liquid medium. Two strains, K-1-1 and K-6-4, were successfully screened by determining the potassium solubilizing ability of phosphorus releasing bacteria and the phosphorus dissolving ability of potassium solubilizing bacteria, which were K-1-1 and K-6-4, respectively. K-1-1 can increase the soluble phosphorus in the medium containing phosphorus by 6.81 渭 g / mL ~ (-1). The strain K-1-1 has a significant advantage over the reported strains of phosphorus-soluble or potassium-free bacteria.
【作者单位】：哈尔滨理工大学化学与环境工程学院/黑龙江省高校绿色化工技术重点实验室;
【基金】：国家自然科学基金资助项目(21406043) 黑龙江省自然科学基金资助项目(C201301) 黑龙江省教育厅重点项目(12541z005)
【分类号】：S154.3

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