库姆塔格沙漠北界阿奇克谷地土壤可培养细菌多样性

发布时间：2018-06-23 08:22

本文选题：阿奇克谷地 + 细菌　；参考：《核农学报》2017年02期

【摘要】：为明确库姆塔格沙漠北界阿奇克谷地的土壤细菌多样性,探索该地区土壤微生物与植物群落的相互作用关系,采用NA、R2A培养基分离培养土壤细菌,通过16S rDNA序列系统发育分析鉴定菌株。结果表明,R2A平板菌落数量和种类较多;发现13株潜在新种菌株,已知菌种分属3大类群、4属、20种,厚壁菌门(Firmicutes)为优势类群,芽胞杆菌属(Bacillus)为优势属,枯草芽胞杆菌(Bacillus subtilis subsp.inaquosorum)为优势菌种。与其他类似环境相比,阿奇克谷地土壤中可培养细菌生物量偏小,可培养的细菌多为抗性、耐性强的极端微生物。该地区特有的微生物资源、功能微生物和新菌资源丰富,为防沙治沙、微生物菌剂开发等研究提供物质基础。本研究结果对库姆塔格沙漠的自然生态保护以及极端环境微生物资源的应用具有重要意义。
[Abstract]:In order to understand the diversity of soil bacteria in Achik Valley, north boundary of Kumtag Desert, and to explore the interaction between soil microbes and plant communities, soil bacteria were isolated and cultured in NAN R2A medium. The strains were identified by phylogenetic analysis of 16s rDNA sequence. The results showed that there were 13 potential new species, including 20 species belonging to 3 groups, 20 species in 4 genera, Firmicutes as the dominant group, Bacillus as the dominant group, and Bacillus as the dominant group. Bacillus subtilis subsp.inaquosorum was the dominant strain. Compared with other similar environments, the biomass of culturable bacteria in the soil of Achik valley was small, and the culturable bacteria were mostly resistant and tolerant extreme microbes. This area is rich in unique microbial resources, functional microorganisms and new bacteria resources, which provides a material basis for the study of sand prevention and control and the development of microbial agents. The results of this study are of great significance to the protection of natural ecology and the application of extreme environmental microbial resources in the Kumtag Desert.
【作者单位】：中国林业科学研究院林业研究所;河北大学生命科学学院;
【基金】：国家林业局948项目(2011-4-71)
【分类号】：S154.3

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