耐盐溶磷菌PSB7的鉴定及其溶磷效果研究

发布时间：2018-06-24 19:48

本文选题：耐盐 + 溶磷菌　；参考：《河南农业科学》2016年07期

【摘要】：通过形态特征、生理生化特征和16S r DNA序列分析对一株分离自黄河三角洲地区沾化冬枣根际土壤的菌株PSB7进行鉴定,并对其耐盐特性和溶磷效果进行测定,为其在提高盐碱化土壤磷素利用率方面的应用提供理论依据。结果表明,菌株PSB7为土壤杆菌(Agrobacterium sp.),在5~35 g/L的Na Cl液体培养基中生长良好。PSB7具有较强的溶磷能力,在改良蒙金娜无机磷液体培养基中培养7 d后,有效磷含量达72.26 mg/L,是无菌株对照的14.54倍。在含盐量0.45%的盐碱化土壤中添加PSB7,培养40 d,土壤中的有效磷含量由68.75 mg/L增加至96.60 mg/L,比未接菌对照增加了40.5%。菌株PSB7兼具耐盐和溶磷特性,在改善盐碱化土壤肥力方面具有很好的应用前景。
[Abstract]:A strain PSB7 isolated from the rhizosphere soil of Zhanhua Jujube in the Yellow River Delta region was identified by morphological, physiological and biochemical characteristics and 16s r DNA sequence analysis. It provides a theoretical basis for improving the utilization rate of phosphorus in salinized soil. The results showed that the strain PSB7 was Agrobacterium (Agrobacterium sp.), which grew well in the NaCl liquid medium of 5 ~ 35g / L. PSB7 had a strong phosphorus solubilizing ability, and was cultured in the modified Montana inorganic phosphorus liquid medium for 7 days. The available phosphorus content was 72.26 mg / L, 14.54 times higher than that of the control. When PSB7 was added to the salinized soil with 0.45% salt content, the available phosphorus content in the soil increased from 68.75 mg / L to 96.60 mg / L for 40 days, which was 40.5% higher than that of the uninoculated control. The strain PSB7 has the characteristics of salt tolerance and phosphorus solubilization, and has a good application prospect in improving soil fertility of salinization.
【作者单位】：滨州学院生命科学系/山东省黄河三角洲野生植物资源开发利用工程技术研究中心;滨州学院资源环境系;
【基金】：山东省高等学校科技计划项目(J10LC55) 滨州学院科研基金项目(2009ZDL03,BZXYFB20110512) 山东省重点研发计划项目(2015GNC111018)
【分类号】：S154.39

【相似文献】