深红虫草高产卵孢菌素的培养基筛选及发酵条件优化
发布时间:2018-06-26 01:00
本文选题:深红虫草 + 卵孢菌素 ; 参考:《菌物学报》2017年04期
【摘要】:本研究以提高深红虫草Cordyceps cardinalis C033次级代谢产物卵孢菌素的产量为目标,对其产卵孢菌素的培养基进行筛选,并利用单因素和正交试验对深红虫草产卵孢菌素的发酵条件进行了优化。结果表明,深红虫草产卵孢菌素的最适发酵培养基为马铃薯 蛋白胨 葡萄糖培养基,最佳发酵参数为:发酵时间6d,培养基初始p H 7.0,接种量6%,培养温度24℃,每500m L锥形瓶装液量100m L,摇瓶转速120r/min。用最佳培养基进行发酵条件优化后获得卵孢菌素448.70μg/m L,产量是优化前的10.14倍。该研究结果为菌株C033在农业害虫防治上的应用奠定了基础。
[Abstract]:The aim of this study was to improve the yield of the secondary metabolite of Cordyceps cardinalis C033, and to screen the culture medium for ovulin, a secondary metabolite of Cordyceps cardinalis C033. The fermentation conditions of oosporin from Cordyceps sinensis were optimized by single factor and orthogonal test. The results showed that the optimum fermentation medium for oosporin of Cordyceps sinensis was potato peptone and glucose medium. The optimum fermentation parameters were as follows: fermentation time 6 days, initial pH 7.0, inoculation amount 6, culture temperature 24 鈩,
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