矿区不同植被复垦模式对土壤细菌群落结构的影响

发布时间：2018-06-28 01:48

本文选题：PCR-DGGE + 复垦矿区　；参考：《环境科学》2017年02期

【摘要】：以安太堡露天煤矿复垦区为研究对象,用PCR-DGGE技术分析不同复垦植被(榆树、落叶松、杏树、云杉和刺槐)及复垦年限(15年和20年)对土壤细菌的影响.土壤细菌多样性分析结果表明:复垦20年组,榆树最高,杏树最低,其余3个植被无显著差异;复垦15年组,云杉显著高于刺槐;刺槐随复垦年限延长,其土壤细菌多样性显著增高,而云杉却反之.相似性系数分析、聚类分析和PCA均显示,相同复垦年限的土样细菌群落结构相似性高.相关性分析表明细菌多样性指数和土壤pH显著正相关.优势和差异条带测序鉴定出Nitrospira、Sphingomonas、Arthrobacter、Brachybacterium、Rhizobium以及Mesorhizobium等或参与氮循环、或降解多环芳烃及杂环有机物的细菌属.本研究说明榆树和云杉有利于土壤细菌多样性的恢复;复垦区土壤的优势菌群多为有利于污染土壤的生态修复和肥力恢复的功能细菌属.
[Abstract]:The effects of different reclaimed vegetation (elm, larch, apricot, spruce and Robinia pseudoacacia) and their reclamation years (15 and 20 years) on soil bacteria were analyzed by PCR-DGGE. The results of bacterial diversity analysis in soil showed that elm was the highest, apricot was the lowest, and the other three vegetation had no significant difference, spruce was significantly higher than Robinia pseudoacacia in reclaimed 15 years, and Robinia pseudoacacia was extended with the years of reclamation. Soil bacterial diversity increased significantly, while spruce did not. Similarity coefficient analysis, cluster analysis and PCA showed that the similarity of bacterial community structure of soil samples with the same reclamation years was high. Correlation analysis showed that bacterial diversity index was positively correlated with soil pH. The dominant and differential bands of Sphingomonas arthrobacter Brachybacterium Brachybacter Rhizobium and Mesorhizobium were identified by sequencing, and the genera of bacteria involved in nitrogen cycling or degradation of polycyclic aromatic hydrocarbons and heterocyclic organic compounds were identified. The results showed that elm and spruce were beneficial to the restoration of soil bacterial diversity, and that the dominant flora of soil in reclamation area was the functional bacteria genus which was beneficial to the ecological restoration and fertility restoration of contaminated soil.
【作者单位】：山西大学生物技术研究所化学生物学与分子工程教育部重点实验室;山西大学黄土高原研究所;
【基金】：国家自然科学基金项目(41271530,31400684) 山西省基础研究项目(2014021030-3) 山西大学引进人才建设项目(206533801001)
【分类号】：S154.3

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