爪哇根结线虫Mj-1-1基因的克

发布时间：2018-06-29 18:52

本文选题：爪哇根结线虫 + Mj--基因　；参考：《植物病理学报》2017年01期

【摘要】：根据爪哇根结线虫食道腺内表达的EST序列,结合反式剪接序列SL1,从爪哇根结线虫中克隆了一个假定寄生基因Mj-1-1(KU358725),该基因的c DNA全长为573 bp,包含450 bp的开放阅读框(ORF),编码149个氨基酸。DNA全长为740 bp,包含2个内含子,长度分别为20 bp和111 bp。NCBI BLASTn比对表明,Mj-1-1与南方根结线虫的M.incognita zk1236.5(JQ284068)基因相似性最高,为97%。原位杂交表明Mj-1-1基因在爪哇根结线虫的背食道腺中表达,qRT-PCR结果表明Mj-1-1基因在爪哇根结线虫寄生性3龄幼虫阶段表达量最高。对爪哇根结线虫侵染前2龄幼虫的Mj-1-1基因进行沉默,调查发现,沉默Mj-1-1后,爪哇根结线虫对番茄的侵染力显著下降,表明Mj-1-1对爪哇根结线虫侵染和寄生具有重要的调控作用。
[Abstract]:According to the EST sequence expressed in the esophagus of Rhizodendron Javanese nematode, Combined with trans-splicing sequence SL1, a hypothetical parasitic gene Mj-1-1 (KU358725) was cloned from the Java root knot nematode. The cDNA of the gene was 573 BP in length and contained an open reading frame (ORF) of 450 BP, encoding 149 amino acids. The total length of DNA was 740 BP, containing two introns. Compared with M. incognita zk1236.5 (JQ284068), Mj-1-1 had the highest similarity with M. incognita (JQ284068), with a length of 20 BP and 111bp.NCBI BLASTn, respectively. In situ hybridization showed that Mj-1-1 gene was expressed in the dorsal esophagus gland of Rhizodendron javanica, and the expression of Mj-1-1 gene was the highest in the parasitic 3rd instar larvae of Rhizodendron javanica. The Mj-1-1 gene of the 2nd instar larvae of Rhizodendron javanica was silenced. It was found that after the silencing of Mj-1-1, the infection of tomato was significantly decreased by Mj-1-1, which indicated that Mj-1-1 played an important role in regulating the infection and parasitism of RKN.
【作者单位】：华南农业大学植物线虫研究室;广东省微生物信号与作物病害防控重点实验室;广东生态工程职业学院;
【基金】：国家重点基础研究发展计划(973计划)(2013CB127501) 国家自然科学基金资助项目(31401716)
【分类号】：S432.45

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