基于高通量测序分析大花杓兰根际土壤细菌多样性

发布时间：2018-06-29 22:17

  本文选题：大花杓兰 + 根际细菌　； 参考：《西南农业学报》2017年04期

【摘要】：【目的】从分子水平上对珍稀濒危药用植物大花杓兰根际土壤细菌群落多样性进行初步探讨。【方法】通过Illumina Miseq高通量测序技术对大花杓兰根际土壤细菌16S rRNA基因的V3~V4高变区片段进行测序,而后进行生物信息学分析。【结果】共得到166,847条16S rRNA基因序列。基于≥97%的相似度水平,通过聚类共获得1549个可操作分类单元(operational taxonomic units,OTUs)。大花杓兰根际土壤细菌在门、纲、目、科和属分类水平上的优势类群分别为变形菌门Proteobacteria(39.67%)、α-变形菌纲α-Proteobacteria(24.72%)、根瘤菌目Rhizobiales(11.11%)、鞘脂单胞菌科Sphingomonadaceae(7.13%)和鞘氨醇单胞菌属Sphingomonas(6.27%)。【结论】首次揭示了大花杓兰根际土壤细菌群落的多样性,为进一步探究大花杓兰与其根际细菌的相互关系以及大花杓兰的迁地保育奠定了基础。
[Abstract]:[objective] to study the diversity of bacterial community in rhizosphere soil of rare and endangered medicinal plant Dipper Magnoliae at molecular level. [methods] the 16s rRNA base of bacteria in rhizosphere soil of Dipper Magnoliae was determined by high throughput sequencing technique of Illumina Miseq. The V3 / V4 hypervariable region fragment was sequenced. Then bioinformatics analysis was carried out. [results] A total of 166847 16s rRNA gene sequences were obtained. Based on the similarity level of 鈮，

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