番茄黄化曲叶病毒的鉴定与群体进化分析

发布时间：2018-07-08 11:29

  本文选题：双生病毒鉴定 + 基因组变异　； 参考：《植物保护》2016年01期

【摘要】：番茄黄化曲叶病(tomato yellow leaf curl disease,TYLCD)是番茄上的重要病害,可由多种双生病毒引起。为了明确北京地区番茄黄化曲叶病的病毒种类和病毒基因组变异情况,本研究收集了51个采自北京不同区县的病毒疑似样品,进行了双生病毒的检测及分离物的基因组分析。经双生病毒简并引物检测25个样品呈阳性;扩增获得10个代表性分离物的全基因组,大小均为2 781bp,共含有6个开放阅读框;经比对,10个分离物与Tomato yellow leaf curl virus-Israel(TYLCV-IL)同源率大于99%;基于我国TYLCV分离物全基因组序列的变异分析表明,TYLCV在进化上比较保守,整个基因组上的变异是不均匀的;系统发育树显示我国TYLCV分离物在进化树上可分为3个组群,分析了我国TYLCV群体进化情况。
[Abstract]:Tomato yellow leaf curl disease (tomato yellow leaf curl disease TYLCD) is an important disease in tomato, which can be caused by a variety of twin viruses. In order to identify the virus species and genomic variation of tomato yellow leaf curl disease in Beijing, 51 suspected virus samples from different districts and counties in Beijing were collected for the detection of the twin virus and the genomic analysis of the isolates. 25 samples were positive by the degenerate primers of Gemini virus, and the whole genome of 10 representative isolates were obtained, which were all 2781 BP in size and contained 6 open reading frames. By comparison, the homology of 10 isolates with Tomato yellow leaf curl virus-Israel (TYLCV-IL) was greater than 99, and the variation analysis of the whole genome sequence of TYLCV isolate from China showed that TYLCV was more conservative in evolution, and the variation of the whole genome was not uniform. Phylogenetic tree showed that TYLCV isolates from China could be divided into three groups on the evolutionary tree. The evolution of TYLCV population in China was analyzed.
【作者单位】： 北京市农林科学院植物保护环境保护研究所;北京农学院植物科学技术学院;
【基金】：公益性行业(农业)科研专项(201003065) 北京市农林科学院青年科研基金(QNJJ 201320)
【分类号】：S436.412.1
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本文编号：2107287