武夷山常绿阔叶林土壤微生物多样性的季节动态

发布时间：2018-07-08 18:39

本文选题：常绿阔叶林 + Illumina　；参考：《热带亚热带植物学报》2017年02期

【摘要】：为了解武夷山自然保护区常绿阔叶林土壤微生物群落特征和季节变化,采用Illumina Miseq高通量测序技术分析了土壤微生物多样性的季节响应。结果表明,武夷山常绿阔叶林土壤为典型的南方酸性土壤,有效钾、土壤温度四季内存在显著差异,其他理化指标均无显著差异。土壤中的微生物多样性比较丰富,已鉴定出23门206属细菌和2门17属的古菌。夏季反映细菌总数的Chao指数最高,但反映细菌多样性的Shannon指数比春季低0.21,夏季古菌的Chao指数和Shannon指数分别比冬季高21.7%和0.27%。4个季节共有的细菌和古菌分别占总数的83.1%和70.0%,说明武夷山常绿阔叶林土壤不同季节的核心微生物组成具有很好的稳定性。在门和属水平的聚类树分析表明,春季和冬季的细菌和古菌群落组成最为接近,而夏季与其他3个季节的差异最大。冗余分析和热图分析结果表明,土壤p H是决定和影响细菌和古菌多样性的主要环境因子,有效钾、有效碳和总氮对微生物群落组成均有很大的影响。因此,随季节变化武夷山常绿阔叶林土壤微生物多样性呈现出规律性变化。
[Abstract]:In order to understand the characteristics and seasonal variation of soil microbial community in evergreen broadleaved forest in Wuyishan Nature Reserve, the seasonal response of soil microbial diversity was analyzed by Illumina Miseq high-throughput sequencing technique. The results showed that the soil of evergreen broad-leaved forest in Wuyi Mountain was a typical acidic soil in southern China. There were significant differences in available potassium and soil temperature in four seasons, but there was no significant difference in other physical and chemical indexes. The diversity of microorganism in soil is rich. 23 phyla 206 genera and 2 phyla 17 genera have been identified. The Chao index, which reflects the total number of bacteria in summer, is the highest. But the Shannon index, which reflects the diversity of bacteria, is 0.21 lower than that in spring. The Chao index and Shannon index of ancient bacteria in summer are 21.7% and 0.27.4% higher than those in winter, respectively. The results indicate that the soil of Wuyi Mountain evergreen broadleaved forest is 83.1% and 70.0% respectively. The core microbial composition of different seasons has good stability. Cluster tree analysis at phylum and genus level showed that the composition of bacteria and palaeomycetes was the most close in spring and winter, but the difference between summer and other three seasons was the greatest. The results of redundancy analysis and thermal map analysis showed that soil pH was the main environmental factor that determined and affected the diversity of bacteria and archaea, and available potassium, available carbon and total nitrogen had great influence on the composition of microbial community. Therefore, soil microbial diversity of evergreen broadleaved forest in Wuyi Mountain changed regularly with seasonal variation.
【作者单位】：福建省农业科学院农业质量标准与检测技术研究所;福建省精密仪器农业测试实验室;福建省武夷山生物研究所;
【基金】：福建省自然科学基金项目(2014J01112) 省属公益类科研院所基本科研专项(2014R1025-8,2016R1024-3) 国家科技支撑计划课题(2013BAC08B03-05)资助~~
【分类号】：S714.3

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