红景天内生放线菌拮抗菌株的筛选及抗药性标记

发布时间：2018-07-10 17:45

本文选题：红景天内生放线菌 + 拮抗　；参考：《云南农业大学学报(自然科学)》2017年05期

【摘要】：【目的】从红景天中挖掘出具有抑菌效果的内生放线菌,为植物病害的生物防治开发新的成员。【方法】从红景天根茎接合部位分离内生放线菌,并采用对峙培养法和菌丝生长速率法筛选对植物病原真菌具有拮抗活性的菌株,测定拮抗活性最强的菌株的16S rRNA基因序列和抑菌谱;同时,为了评估活性最强的菌株与杀菌剂混配使用的可能性,研究了其对常见杀菌剂的抗性。【结果】分离出的19株红景天内生放线菌中有7株具有拮抗活性,其中,Hj3的活性最强且对多数植物病原真菌具有拮抗作用,经16S rRNA基因序列比对初步鉴定为变异链霉菌(Streptomyces variabilis);Hj3在高浓度的啶酰菌胺、多抗霉素、萘啶酸和氨苄西林下均可正常生长。【结论】Hj3在防治常见植物病害方面表现出了一定的优势。
[Abstract]:[objective] to excavate endophytic actinomycetes with bacteriostatic effect from Rhodiola, and to develop a new member for biological control of plant diseases. [methods] Endogenous actinomycetes were isolated from the junction of rhizome of Rhodiola. The strains with antagonistic activity against phytopathogenic fungi were screened by confrontation culture method and mycelium growth rate method, and the 16s rRNA gene sequence and bacteriostasis spectrum of the most antagonistic strains were determined. In order to evaluate the possibility of mixed use of the most active strains and fungicides, the resistance to common fungicides was studied. [results] among the 19 strains of endophytic actinomycetes isolated from Rhodiola, 7 had antagonistic activities. Hj3 has the strongest activity and antagonism to most plant pathogenic fungi. It was identified by 16s rRNA gene sequence alignment as Streptomyces variabilis with high concentration of pyrimidine and polymycin. Both nalidic acid and ampicillin could grow normally. [conclusion] Hj3 has some advantages in controlling common plant diseases.
【作者单位】：忻州师范学院生物系;中国中医科学院中药资源中心道地药材国家重点实验室培育基地;
【基金】：忻州师范学院博士科研启动项目;忻州师范学院科研基金资助项目(201716) 中国中医科学院院级自主选题项目(ZZ2015027)
【分类号】：S476.1

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