玉米茎基腐病生防菌的筛选及应用
发布时间：2018-07-14 15:40
【摘要】：【目的】拟针对禾谷镰孢进行生防菌的筛选和应用研究,以期为玉米茎基腐病生防菌剂的研制奠定基础。【方法】通过对峙培养法对玉米茎基腐病的主要致病菌禾谷镰孢进行玉米内生生防细菌的筛选,从玉米主栽品种九单48幼苗内部获得一株具有较强抑菌效果的内生生防细菌(简称48SJ7-1);基于传统鉴定、16S rRNA基因序列测定及系统发育分析,对48SJ7-1进行鉴定;并通过盆栽试验测定该生防菌株的防效。【结果】菌株48SJ7-1经鉴定为甲基营养型芽孢杆菌,GenBank登录号为KU377993,48SJ7-1盆栽防效为68.47%,与对照药剂2%戊唑醇悬浮种衣剂差异不显著。【结论】48SJ7-1对玉米茎基腐病的主要致病菌禾谷镰孢有较好防效,对玉米生长还有明显的促进作用,而且表观上无药害发生。
[Abstract]:[objective] to study the screening and application of biological control bacteria against Fusarium graminearum, In order to lay a foundation for the preparation of biocontrol agent of maize stem base rot. [methods] screening the bacteria of Fusarium graminearum, the main pathogen of maize stem rot, by means of confrontation culture, An internal bacteriostasis strain (48SJ7-1) with strong bacteriostatic effect was obtained from the seedlings of main maize cultivar Jiudan 48, and 48SJ7-1 was identified based on the sequencing of 16s rRNA gene and phylogenetic analysis. The control effect of the biocontrol strain 48SJ7-1 was determined by pot experiment. [results] the strain 48SJ7-1 was identified as Bacillus spp. The accession number of GenBank was KU377993A48SJ7-1, and the control effect was 68.47%. There was no significant difference between the strain 48SJ7-1 and 2% tebuconazole suspension seed coating agent. [conclusion] 48SJ7-1 has better control effect on Fusarium graminearum, the main pathogen of maize stem base rot. It can also promote the growth of maize, and there is no apparent harm.
【作者单位】： 东北农业大学农学院黑龙江省高校寒地作物品种改良与生理生态重点实验室;
【基金】：国家重点研发计划项目(No.2017YFD0201803) 科技部重点专项项目(No.2016YFD0300704)~~
【分类号】：S435.131.4;S476.1
本文编号：2122122
[Abstract]:[objective] to study the screening and application of biological control bacteria against Fusarium graminearum, In order to lay a foundation for the preparation of biocontrol agent of maize stem base rot. [methods] screening the bacteria of Fusarium graminearum, the main pathogen of maize stem rot, by means of confrontation culture, An internal bacteriostasis strain (48SJ7-1) with strong bacteriostatic effect was obtained from the seedlings of main maize cultivar Jiudan 48, and 48SJ7-1 was identified based on the sequencing of 16s rRNA gene and phylogenetic analysis. The control effect of the biocontrol strain 48SJ7-1 was determined by pot experiment. [results] the strain 48SJ7-1 was identified as Bacillus spp. The accession number of GenBank was KU377993A48SJ7-1, and the control effect was 68.47%. There was no significant difference between the strain 48SJ7-1 and 2% tebuconazole suspension seed coating agent. [conclusion] 48SJ7-1 has better control effect on Fusarium graminearum, the main pathogen of maize stem base rot. It can also promote the growth of maize, and there is no apparent harm.
【作者单位】： 东北农业大学农学院黑龙江省高校寒地作物品种改良与生理生态重点实验室;
【基金】：国家重点研发计划项目(No.2017YFD0201803) 科技部重点专项项目(No.2016YFD0300704)~~
【分类号】：S435.131.4;S476.1
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