纤维素高效降解菌群构建及产酶底物优化
发布时间：2018-07-15 21:56
【摘要】：为促进堆肥腐熟、缩短堆肥周期,通过平板初筛及酶活测定,从腐烂竹子、玉米秸秆中获得4株酶活性较高纤维素降解菌并混合培养,得到理想组合Cro-2/Bam-Q/Bam-3/Bam-1,其内切葡聚糖酶(CX)、外切葡糖酶(C1)、β-外切葡萄糖苷酶(CB)及纤维素全酶(FPA)活性分别为12.54、14.65、7.71、11.98 U,均高于单一菌株酶活性。对混合菌群产酶底物优化结果表明,当沼渣与水稻秸秆11混合,麸皮为氮源时,可有效提高混合菌群产酶活性。
[Abstract]:In order to promote composting maturity and shorten composting cycle, four strains of cellulose-degrading bacteria with high enzyme activity were obtained from decomposed bamboo and corn straw by plate screening and enzyme activity determination. An ideal combination Cro-2 / Bam-Q / Bam-3 / Bam-1 was obtained. The activities of endoglucanase (CX), exoglucinase (C1), 尾 -exoglucinase (CB) and cellulosic holoenzyme (FPA) were 12.54% 14.65.71.71 and 11.98, respectively, which were higher than those of single strain. The results showed that the enzyme production activity of mixed bacteria could be improved effectively when the mixture of biogas residue and rice straw 11 and wheat bran as nitrogen source.
【作者单位】： 东北农业大学工程学院;
【基金】：“十二五”国家科技支撑计划资助项目(2011BAD15B04)
【分类号】：S141.4
本文编号：2125499
[Abstract]:In order to promote composting maturity and shorten composting cycle, four strains of cellulose-degrading bacteria with high enzyme activity were obtained from decomposed bamboo and corn straw by plate screening and enzyme activity determination. An ideal combination Cro-2 / Bam-Q / Bam-3 / Bam-1 was obtained. The activities of endoglucanase (CX), exoglucinase (C1), 尾 -exoglucinase (CB) and cellulosic holoenzyme (FPA) were 12.54% 14.65.71.71 and 11.98, respectively, which were higher than those of single strain. The results showed that the enzyme production activity of mixed bacteria could be improved effectively when the mixture of biogas residue and rice straw 11 and wheat bran as nitrogen source.
【作者单位】： 东北农业大学工程学院;
【基金】：“十二五”国家科技支撑计划资助项目(2011BAD15B04)
【分类号】：S141.4
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