内蒙古大青山森林土壤微生物量碳氮及微生物特征研究
发布时间：2018-07-20 10:20
【摘要】：内蒙古次生林区是我国森林资源的重要组成部分,在我国的生态环境建设以及区域生物地球化学循环中起着非常重要的作用。土壤微生物是森林生态系统的重要组成部分,连接着森林地表植被与地下土壤养分系统,在维持生态系统的平衡过程中起着重要作用。但是到目前为止对内蒙古次生林区土壤碳氮及微生物群落动态的研究甚少。本研究选择大青山常见树种,运用氯仿熏蒸浸提法和实时荧光定量PCR分析方法研究了春秋两季内蒙古呼和浩特市大青山小井沟扁桃(Amygdalus sp.)、油松(Pinus tabulaegormis)、白桦(Betula platyphylla)、山楂(Crataegus pinnatifida)、小檗(Berberisthumbergii)、椴树(Tilia.monolica)、枫树(Acer sp.)、虎榛子(Ostryopsis davidiana)、蒙古栎(Quercus mongolica)、绣线菊(Spiraea sp.)、华北落叶松(Larix principis-rupprechtii)11种根围土壤微生物量碳氮与微生物丰度。研究结果如下：春季不同树种根围土壤微生物量碳变化范围为360mg/kg-984mg/kg,微生物量氮变化范围为63mg/kg-178mg/kg,细菌16S rRNA基因拷贝数变化范围为每克干土中8.8×108-3.6×109个拷贝数,真菌ITS基因拷贝数变化范围为81×107-6.3×108个拷贝数：秋季不同树种根围土壤微生物量碳变化范围为818mg/kg-1342mg/kg,微生物量氮变化范围为217mg/kg-240mg/kg,细菌16S rRNA基因拷贝数变化范围为每克干土中1.7×109-4.1×109个拷贝数,真菌ITS基因拷贝数变化范围为5.8×108-1.7×109个拷贝数。通过结果分析可知,季节和树种对土壤微生物量碳氮与微生物丰度具有显著影响作用,而且,土壤微生物量碳氮含量与土壤微生物丰度显著正相关。
[Abstract]:The secondary forest region of Inner Mongolia is an important part of forest resources in China, which plays an important role in the construction of ecological environment and the regional biogeochemical cycle in China. Soil microorganism is an important component of forest ecosystem, which connects the forest surface vegetation with the soil nutrient system, and plays an important role in the process of maintaining the balance of the ecosystem. However, there are few studies on soil carbon, nitrogen and microbial community dynamics in Inner Mongolia secondary forest. In this study, the common tree species in Daqing Mountain were selected. Chloroform fumigation extraction method and real-time fluorescence quantitative PCR analysis were used to study the effects of spring and autumn on Pinus tabulaegormis), Bai Hua (Crataegus pinnatifida), berberi, Tilia.monolica) and (Acer sp.), tiger hazel of Almond (Pinus tabulaegormis), Bai Hua) in Daqingshan Xiaojinggou, Huhhot City, Inner Mongolia in spring and autumn. 11 species of Spiraea sp.), Larix principis-rupprechtii (Larix principis-rupprechtii) have microbial biomass, carbon, nitrogen and abundance in the rhizosphere soil of Ostryopsis davidiana), (Quercus mongolica), stitch chrysanthemum). The results are as follows: the range of microbial biomass carbon variation is 360 mg / kg-984 mg / kg, microbial biomass nitrogen varies from 63 mg / kg to 178 mg / kg in spring, and the variation range of bacterial 16s rRNA gene copy number is 8.8 脳 108-3.6 脳 109 copies per gram dry soil in spring. The variation range of its gene copy number of fungi is 81 脳 107-6.3 脳 108 copies. The range of microbial biomass carbon variation is 818mg / kg-1342mg / kg, microbial biomass nitrogen variation range is 217mg / kg-240mg / kg, bacterial 16s rRNA gene copy number varies from 1.7 脳 109-4.1 脳 109 copies per gram dry soil in autumn, and the variation range of microbial biomass nitrogen is 217mg / kg-240mg / kg, and the variation range of bacterial 16s rRNA gene copy number is 1.7 脳 109-4.1 脳 109 copies per gram dry soil in autumn. The copy number of its gene varied from 5.8 脳 10 ~ (8) to 1.7 脳 10 ~ (9) copies in fungi. The results showed that season and tree species had significant effects on soil microbial biomass carbon nitrogen and microbial abundance, and soil microbial biomass carbon nitrogen content was positively correlated with soil microbial abundance.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S714.3
本文编号：2133220
[Abstract]:The secondary forest region of Inner Mongolia is an important part of forest resources in China, which plays an important role in the construction of ecological environment and the regional biogeochemical cycle in China. Soil microorganism is an important component of forest ecosystem, which connects the forest surface vegetation with the soil nutrient system, and plays an important role in the process of maintaining the balance of the ecosystem. However, there are few studies on soil carbon, nitrogen and microbial community dynamics in Inner Mongolia secondary forest. In this study, the common tree species in Daqing Mountain were selected. Chloroform fumigation extraction method and real-time fluorescence quantitative PCR analysis were used to study the effects of spring and autumn on Pinus tabulaegormis), Bai Hua (Crataegus pinnatifida), berberi, Tilia.monolica) and (Acer sp.), tiger hazel of Almond (Pinus tabulaegormis), Bai Hua) in Daqingshan Xiaojinggou, Huhhot City, Inner Mongolia in spring and autumn. 11 species of Spiraea sp.), Larix principis-rupprechtii (Larix principis-rupprechtii) have microbial biomass, carbon, nitrogen and abundance in the rhizosphere soil of Ostryopsis davidiana), (Quercus mongolica), stitch chrysanthemum). The results are as follows: the range of microbial biomass carbon variation is 360 mg / kg-984 mg / kg, microbial biomass nitrogen varies from 63 mg / kg to 178 mg / kg in spring, and the variation range of bacterial 16s rRNA gene copy number is 8.8 脳 108-3.6 脳 109 copies per gram dry soil in spring. The variation range of its gene copy number of fungi is 81 脳 107-6.3 脳 108 copies. The range of microbial biomass carbon variation is 818mg / kg-1342mg / kg, microbial biomass nitrogen variation range is 217mg / kg-240mg / kg, bacterial 16s rRNA gene copy number varies from 1.7 脳 109-4.1 脳 109 copies per gram dry soil in autumn, and the variation range of microbial biomass nitrogen is 217mg / kg-240mg / kg, and the variation range of bacterial 16s rRNA gene copy number is 1.7 脳 109-4.1 脳 109 copies per gram dry soil in autumn. The copy number of its gene varied from 5.8 脳 10 ~ (8) to 1.7 脳 10 ~ (9) copies in fungi. The results showed that season and tree species had significant effects on soil microbial biomass carbon nitrogen and microbial abundance, and soil microbial biomass carbon nitrogen content was positively correlated with soil microbial abundance.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S714.3
【参考文献】
相关期刊论文 前1条
1 白军红,邓伟,朱颜明,翟金良,张玉霞;湿地土壤有机质和全氮含量分布特征对比研究——以向海与科尔沁自然保护区为例[J];地理科学;2002年02期
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