甘肃沿黄灌区连作马铃薯根区土壤有机物GC-MS分析

发布时间：2018-07-25 18:53

【摘要】：对轮作(前3 a均种植玉米)和连作(连续4 a种植马铃薯)条件下马铃薯"大西洋"品种出苗期和落花期根区土壤溶液进行GC-MS测定,研究连作逆境下马铃薯根系分泌物的自毒物质。结果发现,两种根区土壤溶液中匹配度80%以上的有机物均检测出烃、苯、酸、醇、酯、醛、酮,酰胺及其烃衍生物,其中出苗期有13种有机物是连作土壤溶液中独有物质,且含量最高的是丙酸丙酯(22.335%),最低的是2-十一烯醛(0.258%)。落花期连作土壤溶液中新增有机化合物7种,其中十六烷酸丙基酯也称棕榈酸丙基酯是植物自毒物质。比对出苗期和落花期两种土壤溶液有机物,确定12种化合物〔壬烷、丙酸丙酯、辛醛、二丁基羟基甲苯、1,2-苯二甲酸二(2-甲基丙基)酯、2,6-二甲基壬烷、2-辛烯醛、2-十一烯醛、1-十二烯、1-十三烷醇、1-十五碳烯、5-十八烯〕不是马铃薯自毒化合物。落花期连作土壤溶液中独有的N,N-二甲基甲酰胺化合物也是出苗期连作土壤独有物质,其浓度为0.455%(出苗期)和0.699%(落花期),该物质是否为自毒物质尚需进一步研究。
[Abstract]:GC-MS was used to determine the soil solution of potato "Atlantic" at seedling emergence and flowering stage under rotation (maize planting in the first 3 years) and continuous cropping (potato growing for 4 years). The autotoxic substances of potato root exudates under continuous cropping stress were studied. The results showed that hydrocarbons, benzene, acids, alcohols, esters, aldehydes, ketones, amides and their hydrocarbon derivatives were detected in soil solutions with matching degree of more than 80%. The highest content was propyl propionate (22.335%), and the lowest was 2-undecenaldehyde (0.258%). Seven new organic compounds were found in continuous cropping soil solution at flowering stage, among which propyl hexadecanoate, also called propyl palmitate, is a plant autotoxic substance. Twelve compounds (nonane, propyl propionate, octylaldehyde) were determined by comparing organic matter in two soil solutions at seedling stage and flowering stage. 2 Ding Ji hydroxyl toluene 1 (2-methylpropyl) bis (2-methylpropyl) dimethylnonane 2-octenal (2-octenal) 1-dodecenol 1-decadecenol 1-pentadecene 5-octadecene) is not an autotoxic compound of potato. N- N- dimethylformamide, which is unique in continuous cropping soil solution at flowering stage, is also unique to continuous cropping soil at emergence stage, and its concentrations are 0.455% (seedling stage) and 0.699% (falling flower period). It is necessary to further study whether the compound is autotoxic.
【作者单位】：甘肃省作物遗传改良与种质创新重点实验室;甘肃条山集团;甘肃农业大学生命科学院;
【基金】：国家科技支撑计划课题(2012BAD06B03)
【分类号】：S153.62

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