亚致死剂量氯吡嘧磺隆胁迫对斑马鱼典型肝脏代谢物的影响

发布时间：2018-07-27 11:02

【摘要】：为获知水环境中亚致死剂量的氯吡嘧磺隆对斑马鱼肝脏的影响,将斑马鱼暴露在亚致死剂量的氯吡嘧磺隆药液中8 d,采用超高效液相色谱与四级杆-轨道离子阱质谱串联对斑马鱼的肝脏代谢物进行分析,采用SIEVE软件将图谱信息转换为含有保留时间、质荷比和相对峰面积的二维数据阵.同时,结合mz Cloud、Human Metabolome Database(HMDB)数据库共鉴定出33种代谢物,包括氨基酸、糖、有机酸等物质.最后,采用SIMCA软件对正离子和负离子模式下的检测结果进行主成分、偏最小二乘法和正交校正的偏最小二乘法分析.主成分和偏最小二乘法结果表明,对照组与处理组在2种检测模式下均存在差异代谢物.为了寻找造成对照组与处理组之间差异的代谢物,对代谢物进行S-Plot分析,结果表明,肌酸酐、牛磺酸、多巴胺和5b-胆甾醇硫酸酯组间差异最大(p0.01).4种代谢产物可作为斑马鱼在氯吡嘧磺隆亚致死剂量胁迫下肝脏代谢异常的潜在标记物.
[Abstract]:In order to know the effect of the lethal dose of clopimuron in water environment on the liver of zebrafish, Zebrafish were exposed to the sublethal dose of clopimuron for 8 days. The liver metabolites of zebrafish were analyzed by ultra-high performance liquid chromatography (HPLC) and four-order rod-orbital ion trap mass spectrometry. SIEVE software is used to transform the map information into a two-dimensional data matrix with retention time, mass charge ratio and relative peak area. At the same time, 33 metabolites, including amino acid, sugar, organic acid and so on, were identified by Metabolome Database (HMDB) database. Finally, the principal component, partial least square method and partial least square method of orthogonal correction are used to analyze the detection results of positive and negative ions using SIMCA software. The results of principal component and partial least square method showed that there were different metabolites between the control group and the treatment group. In order to find the metabolites that caused the difference between the control group and the treatment group, the metabolites were analyzed by S-Plot. The results showed that creatinine, taurine, creatinine, taurine, The largest difference between dopamine and 5b- cholesterol sulfate groups (p0.01) 4 metabolites can be used as potential markers of liver metabolic abnormalities in zebrafish under sublethal dose stress of clopimuron.
【作者单位】：山西农业大学农学院;山西省分析科学研究院;忻州师范学院生物系;
【基金】：山西省植物保护学研究生教育创新项目(No.J201582045)~~
【分类号】：X592

【相似文献】