一种可用于桔小实蝇表皮碳氢化合物分析的固体进样技术

发布时间：2018-07-27 10:36

【摘要】：【目的】利用表皮碳氢化合物进行昆虫鉴定是目前昆虫分类学中非常活跃的研究方向之一。昆虫表皮碳氢化合物的液体浸提及进样技术是GC-MS常规的进样方法之一,随着现代技术的发展,固体进样也被广泛地用于昆虫表皮碳氢化合物的鉴定及昆虫的分类与进化分析。本研究探索利用固体进样法开展实蝇表皮碳氢化合物的提取与分析,探索并获得简便可行的取样与进样技术,为检疫性实蝇的快速鉴定提供新方法。【方法】以桔小实蝇Bactrocera dorsalis(Hendel)成虫为材料,采用固体进样与液体浸提进样方法,开展实蝇表皮碳氢化合物组成成分鉴定与分析;比较获得桔小实蝇成虫取样部位,并分析标本保存时间对桔小实蝇表皮碳氢化合物组成的影响。【结果】利用固体进样技术获得的桔小实蝇翅表皮碳氢化合物种类(39)较液体进样的(36)略多,且翅和中足的表皮碳氢化合物含量分别为0.18~23.37和0.03~4.13 ng/μg,极显著地高于用液体进样法检测到相同部位的化合物含量(分别为0.05~3.93和0.01~0.68 ng/μg)(P0.01),而且前者的检测灵敏度是后者的1.7~10.8倍;同时,翅样品的表皮碳氢化合物含量显著地高于足中的含量(P0.01)。利用固体进样获得的桔小实蝇成虫翅、足或胸部的表皮碳氢化合物图谱与对照处理所得图谱相似。干燥保存30 d的桔小实蝇翅的碳氢化合物的种类及含量与新鲜标本(保存0 d)无显著性差异(P0.05);但保存30,60和150 d标本的碳氢化合物存在显著差异(P0.05),主要是含量的差异。【结论】研究表明,利用固体进样检测昆虫表皮碳氢化合物的灵敏度较高,而且桔小实蝇成虫的主要表皮碳氢化合物种类较稳定,因此可以作为我国口岸检疫性实蝇表皮碳氢化合物获取与分析的一种新方法,而且检测中翅是固体进样的较合适取样部位。
[Abstract]:[objective] to identify insects using epidermal hydrocarbons is one of the most active research directions in insect taxonomy. The liquid extraction and sampling technique of insect epidermis hydrocarbon is one of the conventional methods of GC-MS. With the development of modern technology, solid injection has been widely used in the identification of insect epidermal hydrocarbons and the classification and evolution analysis of insects. In this study, the extraction and analysis of carbohydrates from the epidermis of the fly were carried out by solid sampling method, and a simple and feasible sampling and sampling technique was obtained. To provide a new method for rapid identification of quarantine fruit fly. [methods] using Bactrocera dorsalis (Hendel) adult of fruit fly as material, solid sample and liquid extraction method were used to identify and analyze the composition of hydrocarbon in the epidermis of fruit fly. The sampling sites of adult fruit fly were compared. The effects of preservation time on the composition of carbohydrates in the epidermis of the fruit fly were analyzed. [results] the species of hydrocarbon in the epidermis of the fly wings (39) obtained by solid injection technique were slightly more than those in the liquid sample (36). The contents of hydrocarbon in the epidermis of wing and middle foot were 0.18 渭 g and 0.03 渭 g, respectively, which were significantly higher than those in the same site detected by liquid injection (0.053.93 and 0.01g / 0.68 ng/ g) (P0.01, respectively), and the sensitivity of the former was 1.710.8 times that of the latter, and the sensitivity of the former was 1.710.8 times higher than that of the latter, and the sensitivity of the former was 1.710.8 times higher than that of the latter, and the sensitivity of the former was 1.710.8 times that of the latter. The content of epidermal hydrocarbons in wing samples was significantly higher than that in feet (P0.01). The epidermal hydrocarbon maps of adult wings, feet or chest of orange fruit fly obtained by solid injection were similar to those obtained from the control treatment. There was no significant difference between the species and content of hydrocarbon in the wings of fruit fly preserved for 30 days (P0.05), but there was a significant difference in hydrocarbon content between the specimens of 300.60 and 150 days (P0.05), mainly the difference of the content of hydrocarbon between the fresh specimens (P05) and the fresh specimens (P05). [conclusions] study shows that The sensitivity of solid injection for the detection of insect epidermal hydrocarbons was high, and the main epidermal hydrocarbon species of adult orange-fly were stable. Therefore, it can be used as a new method for obtaining and analyzing epidermis hydrocarbons of quarantine fly in our country, and the detection of middle wing is a more suitable sampling site for solid injection.
【作者单位】：广东省农业科学院植物保护研究所广东省植物保护新技术重点实验室;华南农业大学农学院;广东检验检疫技术中心;
【基金】：广州市科技计划项目(201510010232) 国家科技支撑计划课题(2015BAD08) 科技部科技伙伴计划(KY201402015)
【分类号】：S433

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