黄粉虫虫粉对球孢白僵菌孢子粉产量、质量及其毒力的影响

发布时间：2018-08-04 13:21

【摘要】：球孢白僵菌(Beauveria bassiana)在营养贫乏的培养基质上,菌株易发生退化,为保持高毒力优良菌株的稳定性,采用固液双相法生产球孢白僵菌分生孢子粉,在大米固态培养基中加入不同剂量的黄粉虫虫粉,研究添加虫粉对孢子粉产量、质量及其对大豆食心虫(Leguminivora glycinivorella)毒力的影响。结果表明,添加虫粉对球孢白僵菌的产粉量具有明显的促进作用,其中5.0+处理组的产粉量达到46.88 mg/g;添加虫粉对孢子粉活孢率无显著影响,但各处理组间的萌发速率不同,其中5.0+处理组的孢子萌发率达到50%、90%所需的时间分别为13.86、20.77 h,明显短于对照组。5.0+处理组的孢子粉对大豆食心虫毒力最高,接种后第10天的校正死亡率达到98.27%,侵染率为96.67%,致死中时(lethal time of 50%,简称LT50)为5.58 d。说明添加虫粉可以明显提高大米固态发酵生产球孢白僵菌的孢子粉产量和质量,有利于保持高毒力优良菌株的稳定性。
[Abstract]:The strain of Beauveria bassiana (Beauveria bassiana) is easy to degenerate on the medium with poor nutrition. In order to maintain the stability of the high virulence strain, the conidial powder of Beauveria bassiana was produced by solid-liquid biphasic method. The effects of different dosages of powdery mildew on the yield and quality of sporopollen and its toxicity to (Leguminivora glycinivorella) of soybean heart-eater were studied in rice solid medium. The results showed that the addition of insect powder could obviously promote the production of Beauveria bassiana, and the pollen production of 5.0 treatment group reached 46.88 mg / g, but the germination rate of each treatment group was different. The spore germination rate of the 5.0 treatment group was 13.86 ~ 20.77 h, which was significantly shorter than that of the control group (.5.0), and the spores germination rate of the 5.0 treatment group was the most virulent to soybean heart-eating insects. On the 10th day after inoculation, the corrected death rate was 98.27, and the infection rate was 96.677.The (lethal time of 50 (LT50) was 5.58 days. The results showed that the addition of insect powder could obviously increase the yield and quality of spores powder of Beauveria bassiana produced by solid state fermentation of rice, and it was helpful to maintain the stability of high virulence strains.
【作者单位】：吉林农业大学农学院;
【基金】：吉林省教育厅项目[编号:2013(55)号] 吉林省科学技术厅项目(编号:2007AA021506)
【分类号】：S476.12

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