AM真菌对黄瓜根围土壤微生物群落功能的影响

发布时间：2018-08-06 16:20

【摘要】：在温室土壤灭菌盆栽条件下对黄瓜(Cucumis sativus Linn.,"中农26号")接种丛枝菌根(arbuscular mycorrhizal,AM)真菌Funneliformis mosseae,测定了黄瓜根围土壤微生物功能、微生物数量以及土壤理化性质、土壤酶活性的变化。结果表明AM真菌可以提高土壤的速效养分含量,改善土壤物理性质,提高土壤中磷酸酶、氨肽基酶、多酚氧化酶及过氧化物酶活性;接菌处理后土壤中细菌、真菌数量显著高于不接种处理,而放线菌数量低于不接菌处理;对根围微生物功能多样性指标分析表明接菌处理的平均颜色变化率(AWCD)、Simpson优势度指数以及Mclntosh多样性指数,显著低于不接种对照。对菌根侵染率与土壤微生物等性状间的RDA分析表明AM真菌侵染率与速效P含量(r=0.65,P0.05)、磷酸酶(r=0.74,P0.05)、氨肽基酶(r=0.86,P0.05)、真菌数量(r=0.57,P0.05)呈正相关,与酚酸类碳源(r=-0.55,P0.05)、胺类碳源(r=-0.58,P0.05)、速效N(r=-0.77,P0.05)、β-葡糖苷酶(r=-0.90,P0.05)、β-纤维二糖苷酶(r=-0.63,P0.05)呈负相关。因此接种AM真菌的黄瓜菌根苗种植提高了土壤酶活性,优化了温室黄瓜根围微生物群落结构,显著改善了土壤理化性质。
[Abstract]:Cucumber (Cucumis sativus Linn., Zhongnong 26 was inoculated with arbuscular mycorrhizal AM fungus Funneliformis mosseae under greenhouse soil sterilization. The microbial function, microbial quantity, soil physical and chemical properties and soil enzyme activity were determined. The results showed that AM fungi could increase the content of soil available nutrients, improve the physical properties of soil, increase the activities of phosphatase, aminopeptide enzyme, polyphenol oxidase and peroxidase in soil. The number of fungi was significantly higher than that of non-inoculation treatment, but the number of actinomycetes was lower than that of non-inoculation treatment, and the analysis of microbial functional diversity index showed that the average color variation rate of inoculation treatment was (AWCD) Simpson dominance index and Mclntosh diversity index. It was significantly lower than the control without inoculation. The RDA analysis of mycorrhizal infection rate and soil microorganism showed that the infection rate of AM fungi was positively correlated with the content of available P (0.65%), phosphatase (0.74%), aminopeptidylase (0.86%), and the number of fungi (0.57%). There was a negative correlation between phenolic acid carbon sources (r-0.55U), amine carbon sources (r-0.58U), available N (r-0.77m), 尾 -glucosidase (r-0.90g), 尾 -glucosidase (r-0.63p0.05). Therefore, the cultivation of cucumber root seedlings inoculated with AM fungi increased soil enzyme activity, optimized the microbial community structure of cucumber root circumference in greenhouse, and significantly improved the soil physical and chemical properties.
【作者单位】：中国农业科学院蔬菜花卉研究所;
【基金】：国家重点研发计划项目(2016YFD0201006) 农业部现代农业产业技术体系建设专项资金项目(CARS-25) 中国农业科学院科技创新工程项目(CAAS-ASTIP-2013-IVFCAAS) 农业部园艺作物生物学与种质创制重点实验室项目~~
【分类号】：S154.3

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