猪粪与污泥不同配比对其厌氧共消化与微生物多样性的影响

发布时间：2018-08-09 10:01

【摘要】：研究采用猪粪与城市污水厂脱水污泥以5种不同VS比例(1∶0,2∶1,1∶1,1∶2和0∶1)进行中温厌氧消化实验,以研究反应器在不同配比下的产甲烷特性,同时结合16S rRNA扩增子测序技术分析了消化过程中微生物组成的多样性变化。实验结果表明,添加猪粪能明显提升消化效率,当猪粪与污泥以2∶1混合消化时甲烷累计产量最高可达684L·kg-1VS,比污泥单独消化提升了120%。2∶1组的VS去除率可达63.1%,且运行稳定,没有出现明显的酸抑制现象。随着猪粪的添加,优势菌种演替为Bacteroides、Clostridium、Methanosaeta和Methanosarcina。冗余分析结果表明共消化组中甲烷产生主要以氢营养型途径为主。添加猪粪参与共消化能明显提高微生物群落多样性,促进菌种间的协同作用,从而提升有机质转化效率。
[Abstract]:The experiment of anaerobic digestion at medium temperature with pig manure and dewatered sludge of municipal wastewater treatment plant at 5 different vs ratios (1: 0: 1: 1: 1: 1: 1: 2 and 0:1) was carried out in order to study the methanogenic characteristics of the reactor at different ratios. At the same time, the variation of microbial composition during digestion was analyzed by 16s rRNA Amplifier sequencing. The results showed that the digesting efficiency could be improved obviously by adding pig manure. When the mixture of pig manure and sludge was mixed at 2:1, the accumulative methane production could reach 684L kg -1 VSs, and the removal rate of vs in group 1.2: 1 could reach 63.1% higher than that of sludge digesting alone, and the operation was stable. There was no obvious acid inhibition. With the addition of pig dung, the dominant strains were Bacteroides clostridium Methanosaeta and Methanosarcina. The results of redundancy analysis showed that hydrogen nutrition was the main pathway of methane production in co-digested group. Adding pig manure to co-digestion could significantly improve the diversity of microbial communities and promote the synergy between species, thus enhancing the conversion efficiency of organic matter.
【作者单位】：湖南大学环境科学与工程学院环境生物与控制教育部重点实验室;
【基金】：国家自然科学基金资助项目(51578223,51521006,51378189)
【分类号】：X172;X703;X713

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