典型农田土壤的磷脂脂肪酸分析方法的比较

发布时间：2018-08-09 18:16

【摘要】：以湖北省武穴市典型农田土壤为研究对象,对现有的磷脂脂肪酸(PLFA)提取方法加以改进,分别通过设置不同甲醇萃取时间、不同土壤样品量梯度以及不同正己烷溶解量梯度以确定磷脂脂肪酸提取过程中的甲醇萃取效率、最适土样量和最适溶解量。试验发现,甲醇开始萃取得到的待测样品即为目标样品,用于PLFA提取的武穴典型农田土壤最适提取量为2g,用250μL正己烷溶解的待测样品经检测能够得到有效完整的磷脂脂肪酸数据。为验证改进后的磷脂脂肪酸提取方法的效果,进一步分析了武穴地区种植不同农作物(一季稻、双季稻和油菜)的农田土壤微生物组成,结果表明,该方法的稳定性和重复性良好,能够反映武穴地区典型农田土壤的微生物群落结构。
[Abstract]:Taking the typical farmland soil of Wu-acupoint City in Hubei Province as the research object, the existing methods of (PLFA) extraction of phospholipid fatty acids were improved, and different extraction time of methanol was set respectively. In order to determine the extraction efficiency of methanol, the optimum soil sample quantity and the optimum dissolved amount of phospholipid fatty acid, different soil sample gradient and different n-hexane dissolution gradient were used to determine the extraction efficiency of methanol in the extraction process of phospholipid fatty acids. It was found that the sample to be tested from methanol extraction was the target sample. The optimum extraction amount of the typical farmland soil used for PLFA extraction is 2 g. The available and complete phospholipid fatty acid data can be obtained by detecting the sample dissolved with 250 渭 L n-hexane. In order to verify the effect of the improved extraction method of phospholipid fatty acids, the soil microbial composition of different crops (single cropping rice, double cropping rice and rapeseed) planted in Wuxue area was further analyzed. The method has good stability and reproducibility and can reflect the microbial community structure of typical farmland soil in Wuxue area.
【作者单位】：华中农业大学农业微生物学国家重点实验室;
【基金】：国家重点基础研究发展计划(“973”计划)(2015CB150504) 教育部长江学者和创新团队发展计划项目(IRT1247) 中央高校基本科研业务费专项(2662015PY016,2013PY111和2013PY136)
【分类号】：S154.3

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