扶桑绵粉蚧转录组分析

发布时间：2018-08-19 08:55

【摘要】：【目的】建立扶桑绵粉蚧Phenacoccus solenopsis Tinsley的转录组数据库,揭示扶桑绵粉蚧转录组的整体表达特征。【方法】采用Illumina Hi SeqTM40`00测序平台开展对扶桑绵粉蚧雌成虫转录组测定,对原始数据进行过滤和组装。【结果】共获得58 322 258条序列读取片段(reads),共9.48 Gb(Gen Bank登录号:SAMN06130426)57 422 032条有效转录组数据。进一步组装拼接后,共获得94 475个单基因簇(unigene),平均长度为700 bp。将unigene与数据库中的序列进行BLASTX比对,成功注释20 949个unigenes,其中,Nr注释的unigenes与豌豆蚜Acyrthosiphon pisum unigenes同源性最高,达18.69%。扶桑绵粉蚧转录组unigenes根据GO功能注释大致可分为细胞组分、分子功能和生物过程三大类55个分支,与结合活性、催化活性、细胞进程和代谢进程相关的unigenes较多。此外,本研究还筛选到20条与脂类代谢相关的途径和与性信息素代谢相关的序列。并通过与Nr和Swiss-prot蛋白质数据库比对,获得了15 037条编码序列(CDS)片段。【结论】本研究初步阐明扶桑绵粉蚧雌成虫转录组的整体表达模式,为进一步研究扶桑绵粉蚧的基因功能及性信息素的代谢途径奠定了基础。
[Abstract]:[objective] to establish the transcriptional database of Phenacoccus solenopsis Tinsley and reveal the overall expression characteristics of the transcriptome. [methods] the Illumina Hi SeqTM40 `00 sequencing platform was used to detect the transcriptional structure of female adults. The original data were filtered and assembled. [results] A total of 58,322,258 (reads), sequences were obtained, which included 9.48 Gb (Gen Bank accession number: SAMN06130426) 57,422,032 effective transcriptional group data. The average length of (unigene), of 94,475 single gene clusters was 700 BP. The sequence of unigene was compared with the sequence in the database, and 20 949 unigenes were successfully annotated, among which the unigenes annotated by Nr had the highest homology with the Acyrthosiphon pisum unigenes of the pea aphid, reaching 18.69. According to go functional annotation, the transcription group unigenes can be divided into 55 branches, including cell component, molecular function and biological process. There are more unigenes related to binding activity, catalytic activity, cellular process and metabolic process. In addition, 20 pathways related to lipid metabolism and sequences related to sex pheromone metabolism were screened. By comparing with Nr and Swiss-prot protein database, 15 037 coding sequence (CDS) fragments were obtained. [conclusion] the whole expression pattern of the transcriptional group of female adult insects was preliminarily elucidated in this study. It lays a foundation for further study on gene function and metabolic pathway of sex pheromone.
【作者单位】：广州大学生命科学学院;广东省生物资源与应用研究所;
【基金】：广州市属高校科技计划项目(1201430778) 2016年度广州大学大学生创新训练项目(201611078064)
【分类号】：S433

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