CgRGS7调控胶孢炭疽菌分生孢子产量、附着胞形成及致病性
发布时间：2018-08-20 10:01
【摘要】：【目的】G蛋白信号调控因子(regulators of G-protein signaling,RGS)是G蛋白信号转导通路中的负调控因子,参与多个G蛋白信号通路介导的细胞内过程,目前对于胶孢炭疽菌相关RGS蛋白的生物学功能研究较少。【方法】本研究通过同源重组获得CgRGS7基因的敲除突变体,并对其生物学功能进行初步分析。【结果】CgRGS7基因编码620个氨基酸,具有7个跨膜结构域和1个RGS功能域。CgRGS7敲除突变体与野生型菌株相比,表现为分生孢子产量降低且孢子呈多端萌发,附着胞形成率下降以及致病性减弱。【结论】CgRGS7参与调控胶孢炭疽菌分生孢子产量,同时影响芽管的形态发育、附着胞形成及致病性。
[Abstract]:[objective] G protein signal regulator (regulators of G-protein signalingr GS is a negative regulation factor in G protein signal transduction pathway and participates in many intracellular processes mediated by G protein signaling pathway. There are few studies on the biological function of RGS proteins related to anthracis. [methods] the knockout mutants of CgRGS7 gene were obtained by homologous recombination. [results] the CgRGS7 gene encodes 620 amino acids, and has 7 transmembrane domains and 1 RGS functional domain. CgRGS7 knockout mutants are compared with wild-type strains. The results showed that the conidial yield decreased, the spores germinated at multiple ends, the appressant formation rate decreased and the pathogenicity weakened. [conclusion] CgRGS7 was involved in the regulation of conidial yield of Bacillus anthracis and the morphological development of bud tube. Attachment formation and pathogenicity.
【作者单位】： 海南大学热带农林学院;
【基金】：国家自然科学基金(31560045) 海南省重点研发计划(ZDYF2016155)
【分类号】：S432.44
本文编号：2193221
[Abstract]:[objective] G protein signal regulator (regulators of G-protein signalingr GS is a negative regulation factor in G protein signal transduction pathway and participates in many intracellular processes mediated by G protein signaling pathway. There are few studies on the biological function of RGS proteins related to anthracis. [methods] the knockout mutants of CgRGS7 gene were obtained by homologous recombination. [results] the CgRGS7 gene encodes 620 amino acids, and has 7 transmembrane domains and 1 RGS functional domain. CgRGS7 knockout mutants are compared with wild-type strains. The results showed that the conidial yield decreased, the spores germinated at multiple ends, the appressant formation rate decreased and the pathogenicity weakened. [conclusion] CgRGS7 was involved in the regulation of conidial yield of Bacillus anthracis and the morphological development of bud tube. Attachment formation and pathogenicity.
【作者单位】： 海南大学热带农林学院;
【基金】：国家自然科学基金(31560045) 海南省重点研发计划(ZDYF2016155)
【分类号】：S432.44
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