氮肥减量配施微生物菌剂对灌淤土花椰菜产量及土壤微生物的影响

发布时间：2018-08-23 12:35

【摘要】：采用biolog微生物鉴定系统和平板稀释法探讨了氮肥减量配施自制微生物菌剂对花椰菜产量及根际土壤微生物的影响研究。结果表明:T4(2/3倍N肥用量+微生物菌剂)、T5(3/4倍N肥用量+微生物菌剂)和T6(常规N肥用量+微生物菌剂)处理土壤细菌、放线菌和微生物总数数量分别增加了57.6%~321.54%,66.81%~83.62%和135.40%~232.07%,而土壤真菌数量降低34.08%~41.80%。与对照相比各施肥处理增加了土壤细菌占微生物总数的比例,降低了真菌和放线菌的比例,T5和T4处理效果最显著。与对照相比T4,T5和T6处理显著提高了土壤微生物群总落碳源利用能力。T4,T5和T6处理显著增加了土壤微生物群落对碳水化合物、羧酸类、聚合物类和氨基酸类碳源的代谢水平,T3(单施微生物菌剂)处理显著增加了土壤微生物群落对羧酸类和聚合物类碳源的代谢水平。T5和T6处理对增加花椰菜产量、生物量效果接近,花椰菜产量分别增加了12.35%和10.75%,地下干生物量增加了57%左右,T2(单施氮肥)和T4处理增加花椰菜产量5%左右。
[Abstract]:Biolog microbiological identification system and plate dilution method were used to study the effects of nitrogen fertilizer reduction and self-made microbial agents on the yield of cauliflower and rhizosphere soil microbes. The results showed that T5 (3 / 4 times N) and T6 (routine N fertilizer) were used to treat soil bacteria. The total number of actinomycetes and microbes increased by 57.6% and 66.81%, respectively, and the amount of soil fungi decreased by 34.08% and 41.80%, respectively, and the total number of actinomycetes and microorganisms increased by 83.62% and 135.402.07%, respectively. Compared with the control, the fertilization treatments increased the proportion of soil bacteria to the total number of microorganisms, and reduced the ratio of fungi and actinomycetes to T5 and T4 treatments. Compared with the control, the treatments of T4, T5 and T6 significantly increased the total carbon source utilization capacity of soil microbial community. T4, T5 and T6 treatments significantly increased the carbohydrates and carboxylic acids in soil microbial communities. Metabolic level of carbon sources of Polymer and Amino acids (TT3) treatment significantly increased the metabolic level of soil microbial community to carboxylic acid and polymer carbon sources. T5 and T6 treatments increased the yield of cauliflower, and the biomass effect was similar. The yield of cauliflower increased by 12.35% and 10.75%, and the dry biomass increased by 57% or so by T _ 2 and T _ 4 treatments, respectively, and increased the yield of cauliflower by about 5%.
【作者单位】：宁夏农林科学院农业资源与环境研究所;
【基金】：宁夏农林科学院自主研发项目(NKYG-15-15;NKYJ-16-03) 宁夏自然科学基金项目(NZ14189) 国家自然科学基金项目(41661066)
【分类号】：S154.3;S635.3

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