锯末牛粪堆肥微生物多样性的宏基因组学分析

发布时间：2018-09-13 09:54

【摘要】：运用宏基因组技术研究不同比例锯末牛粪堆肥产物中微生物的组成和分布。结果表明,牛粪与锯末质量比为2∶1、3∶1、4∶1的3个堆肥样本中微生物可操作分类单元(operational taxonomic units,OTU)有较大的差异,其中含量最高的3个门的微生物为变形菌门、拟杆菌门和厚壁菌门,在不同样本中它们的含量也有较大差异。从属水平分析,含量最高的是甲基单胞菌属、噬氢菌属、固氮菌属和寡养单胞菌等营养转化和合成的菌属。降解纤维素和木质素的也有一定的含量,特别是假单胞菌属含量较高。经过对堆肥产物基本性质及其微生物的分析发现,当牛粪与锯末的比例为2∶1时,堆肥样品中的营养转化和合成菌属以及去除污染菌属都高于其他2个样品,堆肥效果较好。
[Abstract]:The composition and distribution of microorganisms in different proportion of sawdust cattle manure compost were studied by macrogenome technique. The results showed that there was significant difference among the three compost samples with the mass ratio of cow dung to sawdust of 2: 1: 3: 1: 4: 1. The microbial operable taxonomy unit (operational taxonomic units,OTU) of the three compost samples with the highest content was Proteus. The content of Bacteroides phylum and phylum thuringiensis in different samples were also different. According to the analysis of subordinate level, the highest content is the genus Methylomonas, Hydrocytoma, Azotobacter and Oligotrophomonas. The content of cellulose and lignin was also high, especially in Pseudomonas. By analyzing the basic properties of compost products and their microbes, it was found that when the ratio of cow manure to sawdust was 2:1, the genera of nutrient transformation, synthetic bacteria and decontamination bacteria in compost samples were higher than those of the other two samples, and the composting effect was better.
【作者单位】：文华学院环境工程系;
【基金】：湖北省省级战略新兴(支柱)产业人才培养计划——环境工程(编号:j0900760102)
【分类号】：S141.4

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