1株甘蔗根际促生细菌的鉴定及其生物学功能研究初报

发布时间：2018-10-07 17:40

【摘要】：为了给甘蔗根际促生细菌的开发和利用提供理论依据,以及对微生物功能基因的挖掘利用提供有价值的菌种资源。利用常规分离方法从甘蔗根际中分离获得菌株GG08160,通过固氮酶活性测定、固氮酶基因nif H扩增及序列分析鉴定该细菌的固氮能力;采用Salkowski比色法和溶磷圈D/d比值方法分别测定该菌株分泌IAA以及对无机磷的降解能力,利用含有浓度梯度草甘膦的培养基胁迫培养测定该菌株对草甘膦的抗性;采用盆栽接种法明确菌株对甘蔗幼苗的实际促生长作用;根据形态学、Biolog检测和16S rRNA序列分析对菌株GG08160进行鉴定,确定该菌株的分类地位。研究结果表明,菌株GG08160具有固氮、分泌IAA和降解无机磷的功能,能显著促进甘蔗幼苗的生长;该菌株在草甘膦含量达250 m M的培养基质中仍能正常生长,具有高抗草甘膦的活性;根据菌株培养性状、Biolog检测和16S r DNA序列分析结果,把该菌株鉴定为克雷伯氏菌属(Klebsiella sp.)细菌。
[Abstract]:In order to provide theoretical basis for the development and utilization of sugarcane rhizosphere growth-promoting bacteria, and to provide valuable resources for the exploitation and utilization of microbial functional genes. The strain GG08160, was isolated from the rhizosphere of sugarcane by routine isolation method. The nitrogenase activity was determined by nif H amplification and sequence analysis of the nitrogenase gene. The Salkowski colorimetric method and the D- / d ratio method were used to determine the ability of IAA secretion and inorganic phosphorus degradation of the strain. The resistance of the strain to glyphosate was determined by stress culture medium containing concentration gradient glyphosate. Pot inoculation method was used to determine the actual growth promoting effect of the strain on sugarcane seedlings, and to identify the strain GG08160 according to morphological Biolog detection and 16s rRNA sequence analysis, and to determine the taxonomic position of the strain. The results showed that the strain GG08160 had the functions of nitrogen fixation, secretion of IAA and degradation of inorganic phosphorus, and could promote the growth of sugarcane seedlings significantly, and the strain could still grow normally in the medium with glyphosate content up to 250mm. The strain was identified as Klebsiella (Klebsiella sp.) by Biolog detection and 16s r DNA sequence analysis. Bacteria.
【作者单位】：广西农业科学院微生物研究所;广西作物遗传改良生物技术重点开放实验室;中国农业科学院甘蔗研究中心;广西大学;
【基金】：国家自然科学基金项目(31101122,31171504) 广西农业科学院基金项目(2013YT04,2013JQ09,2014GW07) “广西特聘专家”专项
【分类号】：S566.1;S182

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