氮肥对盐渍化枸杞园土壤微生物群落和氨氧化微生物丰度的影响
发布时间：2018-10-08 10:59
【摘要】：研究主要分析氮元素对宁夏平罗盐渍化枸杞园土壤中氨氧化微生物(氨氧化细菌和氨氧化古菌)的影响。实验共设八个处理:(1)C(不施氮肥,不施脱硫废弃物,原始荒地);(2)不施氮肥(N0);(3)施氮肥25 kg hm~(-2)(N25);(4)施氮肥50 kg hm~(-2)(N50);(5)施氮肥100kg hm~(-2)(N100);(6)施氮肥200 kg hm~(-2)(N200);(7)施氮肥400 kg hm~(-2)(N400);(8)施氮肥800 kg hm~(-2)(N800),在N0-N800处理施用脱硫废弃物3.72×104kg hm~(-2)。2011年8月采集0~20 cm土样。结果显示:脱硫废弃物和氮肥配合施加对土壤理化性质产生了显著影响;NO_3~--N和NH_4~+-N含量在施氮处理中相对于原始样地和不施氮处理组都有显著的升高,微生物生物量和细菌和氨氧化细菌多样性指数在施氮400 kg hm~(-2)达到最大值,施氮肥400 kg hm~(-2)是促进微生物量和群落多样性增加的最佳施用量。实时荧光定量PCR结果显示:氨氧化细菌(AOB)丰度在施氮肥400 kg hm~(-2)和800 kg hm~(-2)显著高于其它处理,脱硫废弃物和氮肥配合施用对AOB的丰度具有叠加效应。相关性分析表明:NH_4~+-N与总磷脂脂肪酸(PLFA)、细菌PLFA、革兰氏阳性菌(G+)、革兰氏阴性菌(G-)、真菌/细菌(F/B)、微生物碳(MBC)、微生物氮(MBN)及16S r RNA基因拷贝数、AOB的基因拷贝数都显著相关。因此,铵态氮是该地区微生物群落可利用的有效氮素。
[Abstract]:The effect of nitrogen on ammonia-oxidizing microorganisms (ammonia-oxidizing bacteria and ancient ammonia-oxidizing bacteria) in the soil of Pingluo salinized Lycium barbarum orchard in Ningxia was studied. There were eight treatments in the experiment: (1) C (without nitrogen fertilizer, no desulfurization waste, 鍘熷鑽掑湴);(2)涓嶆柦姘偉(N0);(3)鏂芥爱鑲，
本文编号：2256501
[Abstract]:The effect of nitrogen on ammonia-oxidizing microorganisms (ammonia-oxidizing bacteria and ancient ammonia-oxidizing bacteria) in the soil of Pingluo salinized Lycium barbarum orchard in Ningxia was studied. There were eight treatments in the experiment: (1) C (without nitrogen fertilizer, no desulfurization waste, 鍘熷鑽掑湴);(2)涓嶆柦姘偉(N0);(3)鏂芥爱鑲，
本文编号：2256501
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