一株杀扑磷高效降解菌的分离、鉴定及降解条件的响应面法优化
发布时间：2018-10-09 10:45
【摘要】：有机磷杀虫剂杀扑磷对防治蚧壳虫有特效,常用于柑橘类果树防治但其残留有毒有害,自然界降解速度比较缓慢.本研究分离并分类鉴定了能高效降解杀扑磷的菌株,进一步采用Box-Benhnken法设计3因素3水平的响应面试验优化降解条件.结果显示,从富集培养基中分离到了一株杀扑磷高效降解细菌MS1-2,经鉴定为膝形假单胞菌(Pseudomonas geniculata);单因素选择温度、NaCl浓度和pH,通过检测杀扑磷残留量对杀扑磷降解条件进行响应面优化,转速设置为100 r/min,接种量为0.4%时,温度和Na Cl浓度为主要影响因素,pH为次要因素,最佳降解条件为温度29.74℃,pH 6.48,Na Cl浓度0.63%.在此条件下培养24 h,杀扑磷从100 mg/L降解到57.73 mg/L,降解率为42.27%,比优化前提高了6个百分点.因此菌株MS1-2能有效降解杀扑磷,优化降解条件后降解率有所提高.
[Abstract]:Organophosphorus insecticide has a special effect on the control of scale insects. It is often used to control citrus fruit trees, but its residue is poisonous and harmful, and the natural degradation rate is slow. In this study, we isolated and classified the strains that could efficiently degrade triaphos, and further optimized the degradation conditions by using the Box-Benhnken method to design 3 factors and 3 levels of response surface test. The results show that A highly efficient biodegradable bacterium MS1-2, was isolated from the enrichment medium. It was identified as Pseudomonas geniculata (Pseudomonas geniculata); univariate selection temperature and pH, concentration, and the response surface was optimized by detecting the residual amount of propofol. Temperature and Na Cl concentration were the main influencing factors and pH was the secondary factor when the rotational speed was set at 100r / min and inoculation volume was 0.40.The optimum degradation conditions were as follows: temperature 29.74 鈩，
本文编号：2259049
[Abstract]:Organophosphorus insecticide has a special effect on the control of scale insects. It is often used to control citrus fruit trees, but its residue is poisonous and harmful, and the natural degradation rate is slow. In this study, we isolated and classified the strains that could efficiently degrade triaphos, and further optimized the degradation conditions by using the Box-Benhnken method to design 3 factors and 3 levels of response surface test. The results show that A highly efficient biodegradable bacterium MS1-2, was isolated from the enrichment medium. It was identified as Pseudomonas geniculata (Pseudomonas geniculata); univariate selection temperature and pH, concentration, and the response surface was optimized by detecting the residual amount of propofol. Temperature and Na Cl concentration were the main influencing factors and pH was the secondary factor when the rotational speed was set at 100r / min and inoculation volume was 0.40.The optimum degradation conditions were as follows: temperature 29.74 鈩，
本文编号：2259049
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